Expression of cytochrome P450 17alpha-hydroxylase/C17-20 lyase in the fetal rat testis is reduced by maternal exposure to exogenous estrogens

Endocrinology
G MajdicP T Saunders

Abstract

Testosterone is required for normal development of the male reproductive tract. Synthesis of testosterone occurs in the Leydig cells and is dependent upon the expression of several enzymes, including cytochrome P450 17alpha-hydroxylase/C17-20-lyase (P450c17), which is highly regulated within the testis. The aim of the present study was to investigate whether maternal exposure to estrogenic chemicals was able to affect Leydig cell function in the developing male fetus at the time of masculinization. Pregnant rats were injected sc with diethylstilbestrol (DES; 100 or 500 micrograms/kg), 4-octylphenol (OP; 100 or 600 mg/kg), or vehicle (oil, control) on days 11.5 and 15.5 postcoitum. Doses were chosen to reflect the reported estrogenic potency of the chemicals in vitro. On day 17.5, fetal testes were fixed before performing in situ hybridization and immunocytochemistry, used for extraction of RNA, or homogenized in phosphate buffer for determination of 17alpha-hydroxylase enzyme activity. There was no difference between fetuses from control and treated mothers in either the overall histology of the testes or the apparent number of Leydig cells, as determined by immunocytochemistry with an antibody directed against 3beta-hydroxyste...Continue Reading

Citations

Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·R SleeH J Cooke
Sep 14, 2010·The Journal of Endocrinology·Eugenia H GouldingEdward M Eddy
Jun 16, 2000·Molecular and Cellular Endocrinology·V SriramanA J Rao
Aug 14, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y IkedaS Hayashi
Jun 16, 2004·Molecular and Cellular Endocrinology·Romain GuyotBrigitte Le Magueresse-Battistoni
Oct 31, 2012·Toxicology and Industrial Health·Jianying YangCuili Qin
Jun 22, 2000·Biology of Reproduction·H B Siril AriyaratneJ Ian Mason
Sep 30, 2016·Reproduction, Fertility, and Development·P A AulerF R C L Almeida
Oct 4, 2007·Journal of Biochemical and Molecular Toxicology·Lotte N MoensW M De Coen
Oct 23, 2002·Environmental Toxicology and Chemistry·Sirinmas KatchamartDonald R Buhler
Jan 14, 1999·Endocrinology·A M van PeltG G Kuiper
Mar 16, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tiina F M MikkiläJorma Paranko
May 20, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jorge M NaciffGeorge P Daston
Sep 18, 2004·Endocrinology·Matthew P Hardy, Peter N Schlegel
Jan 22, 2003·International Journal of Andrology·Richard M Sharpe

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.