Expression of cytochrome P4502E1 in human liver: Relationship between genotype and phenotype in Chinese

Science in China. Series C, Life Sciences
W TanD Lin

Abstract

Polymorphic cytochrome P4502E1 (CYP2E1) plays an important role in the metabolic activation of many carcinogens. We have previously shown that the c1/c1 genotype recognized by Rsa I in the 5'-regulatory region of the CYP2E1 may be a susceptibility factor for developing esophageal cancer and lung cancer in Chinese. The present study was to investigate the relationship between the Rsa I genotype and the expression of CYP2E1 in human livers. A total of 50 liver specimens were genotyped for CYP2E1 and assayed for CYP2E1 protein contents and functional activity by using specific antibody in immunoblot and a probe substrate,p-nitrophenol. A considerable interindividual variation in CYP2E1 protein (20-fold) and functional activity (56-fold) was observed among these liver samples. However, when they were categorized according to genotype, the mean content of CYP2E1 protein was significantly higher among individuals with the c1/c1 genotype than that among those having c1/c2 or c2/c2 genotype [124.0+/-83.9 pmol/mg (n = 28) versus 65.5 +/-38.9 pmol/mg (n = 22),P<0.01]. The mean activity of CYP2E1 towards p-nitrophenol for the c1/c1 genotype was also higher than that for the variant genotypes (198.4+/-27.8 pmol/min/mg versus 101.2 +/-18.1 ...Continue Reading

References

Mar 1, 1991·Chemical Research in Toxicology·F P GuengerichM Iwasaki
Dec 1, 1989·European Journal of Clinical Investigation·N PerrotP H Beaune
Jan 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J GeorgeG C Farrell
Feb 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T TakahashiC S Lieber
Jun 1, 1996·Pharmacogenetics·V CarrièreI de Waziers

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Citations

Dec 25, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Yadong WangBaoyu Zhu
Jul 22, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Ya-Dong WangHai-Yu Wang
Jan 25, 2020·Molecular Medicine Reports·Rui-Ying WangXiao-Bing Shen

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