Expression of cytosolic phospholipase A2 in equine endometrium during the oestrous cycle and early pregnancy

Reproduction in Domestic Animals = Zuchthygiene
Mm AbabnehF Shidaifat

Abstract

Phospholipase A2 (PLA2) is a key enzyme for biosynthesis of PGF2α. Real-time RT-PCR and immunohistochemistry were used to determine transcription and cellular distribution of cytosolic PLA2 (cPLA2) in the equine endometrium during the oestrous cycle and early pregnancy. Endometrial biopsy and blood samples were collected from cycling mares on days (d) 8, 11, 15 and 18 (oestrus) (Day 0 = Day of ovulation; n = 5 for each day) and from pregnant mares (n = 4) on d15. Except for mares on d18 and some cyclic mares (n = 2) on d15 with low progesterone (P₄) concentrations (< 3.18 nm), P₄ concentrations were high. Cytosolic PLA2 was mainly localized in the luminal epithelium and stroma was negative. Cytosolic PLA2 expression was negatively correlated with P₄ concentration (r = -0.75, P < 0.001) and differed according to the stage of the oestrous cycle (P < 0.05). Cytosolic PLA2 expression was high during oestrus and declined to basal levels on d8 (P < 0.05). Thereafter, there was a trend for increased cPLA2 expression as luteal phase progressed. However, as P₄ dropped below 3.18 nm on d15, cPLA2 expression increased (P < 0.05). In pregnant mares, cPLA2 expression was not different from cyclic mares on d15 with high P₄ concentrations. Ho...Continue Reading

References

Jan 1, 1987·Journal of Steroid Biochemistry·R C BonneyS Franks
Dec 1, 1983·Prostaglandins·P L PakrasiS K Dey
Jan 1, 1997·Reproduction, Fertility, and Development·M A KaminskiW J Silvia
Jun 14, 2000·Animal Reproduction Science·D C Sharp
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 21, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·B A QuinnK J Betteridge
Jul 25, 2008·Reproduction in Domestic Animals = Zuchthygiene·M A HayesK J Betteridge

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Citations

Apr 11, 2012·Reproduction in Domestic Animals = Zuchthygiene·M M Ababneh, M H T Troedsson
Aug 14, 2012·Reproduction in Domestic Animals = Zuchthygiene·M M Ababneh, M H T Troedsson
Dec 1, 2011·Reproduction, Fertility, and Development·C Klein, M H T Troedsson
Nov 1, 2013·Biology of Reproduction·Anastazia SamborskiStefan Bauersachs
Oct 7, 2015·Journal of Animal Science·K M KlohonatzJ E Bruemmer

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