PMID: 9177309May 1, 1997Paper

Expression of de novo high-lysine alpha-helical coiled-coil proteins may significantly increase the accumulated levels of lysine in mature seeds of transgenic tobacco plants

Plant Molecular Biology
S J KeelerJ A Rice

Abstract

We have designed protein molecules based on an alpha-helical coiled-coil structure. These proteins can be tailored to complement nutritionally unbalanced seed meals. In particular, these proteins may contain up to 43% mol/mol of the essential amino acid lysine. Genes encoding such proteins were constructed using synthetic oligonucleotides and the protein stability was tested for in vivo by expression in an Escherichia coli model system. A protein containing 31% lysine and 20% methionine (CP 3-5) was expressed in transgenic tobacco seeds utilizing the seed specific bean phaseolin and soybean beta-conglycinin promoters. Both promoters provided a level of expression in the mature transgenic tobacco seeds which resulted in a significant increase in the total lysine content of the seeds. Several of these transgenic lines were analyzed for three generations to determine the stability of gene expression. Plants transformed with the soybean beta-conglycinin promoter/CP 3-5 gene consistently expressed the high-lysine phenotype through three generations. However, expression of the high-lysine phenotype in plants transformed with the bean phaseolin/CP 3-5 was variable. This is the first report of a significant increase in seed lysine cont...Continue Reading

Citations

Aug 23, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G LackUNKNOWN BSACI working party
Jul 28, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Elizabeth S DennisW J Peacock
Jul 13, 2016·Journal of Experimental Botany·Wenyi Wang, Gad Galili
Mar 2, 2018·Protein Science : a Publication of the Protein Society·Radhika P NagarkarJoel P Schneider
Jan 18, 2014·Transgenic Research·Yunfang ZhangDaina H Simmonds
Apr 1, 2010·Biochimie·Tatiana P Soares da CostaJuliet A Gerrard

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