Expression of delta-lactoferrin induces cell cycle arrest

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Mathilde BretonAnnick Pierce

Abstract

Delta-lactoferrin (deltaLf) mRNA is the product of alternative splicing of the Lf gene. It has been found in normal tissues and was reported to be absent from their malignant counterparts. Our recent investigations have shown that deltaLf expression is a good prognostic indicator in human breast cancer. However, deltaLf has up till now only been identified as a transcript, and in order to characterize the deltaLf protein and determine its function we have used a deltaLf cDNA construct to produce the protein in vitro and in vivo. A 73 kDa protein was immunoprecipitated from in vitro translation products and this molecular weight is in accordance with the use of the first in frame AUG start codon located in exon 2. We also produced a cell line expressing deltaLf under doxycycline induction. Using this model we have been able to show that deltaLf is mainly distributed in the cytoplasm. Its expression induces cell cycle arrest and inhibits cell proliferation. Our results suggest that deltaLf may play an important role in the regulation of normal cell growth.

Citations

Apr 21, 2010·The Journal of Biological Chemistry·Stéphan HardivilléAnnick Pierce
Dec 19, 2008·Critical Reviews in Food Science and Nutrition·Lígia RodriguesHelena Lindmark Månsson
Feb 11, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Christophe MarillerAnnick Pierce
Jul 25, 2013·Critical Reviews in Oncogenesis·Suzy V Torti, Frank M Torti
May 16, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Stéphan HardivilléAnnick Pierce
Nov 16, 2004·International Journal of Cancer. Journal International Du Cancer·Monique BenaïssaAnnick Pierce
Jan 24, 2009·The American Journal of Pathology·Federica SotgiaMichael P Lisanti
Nov 20, 2014·Nutrition Reviews·Yunlei ZhangLigia R Rodrigues
Oct 31, 2014·Nutrition and Cancer·Yunlei ZhangLígia R Rodrigues
Jun 26, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Matthias DierickEric Cox

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