Expression of differentiation-associated gene icb-1 is estrogen-responsive in ovarian and breast cancer cell lines

The Journal of Steroid Biochemistry and Molecular Biology
Julia BollmannOliver Treeck

Abstract

icb-1 (C1orf38) is a human gene initially described by our group to be upregulated during in vitro differentiation processes of endometrial adenocarcinoma and leukemia cells triggered by different stimuli. We now report presence of a putative imperfect estrogen response element (ERE) in the promoter of icb-1 gene. Given that estrogens are known to regulate cellular differentiation processes of hormone-dependent tissues, we studied whether expression of icb-1 would be regulated by 17-beta (beta) estradiol in breast and ovarian cancer cells. As examined by means of real time PCR, treatment with 17-beta estradiol for at least 24h resulted in a significant increase of icb-1 transcript levels in ERalpha-positive MCF-7 breast cancer and OVCAR-3 ovarian cancer cells, but not in ERalpha-negative SK-BR-3 and SK-OV-3 cells. Upregulation of icb-1 transcript levels was also observed after treatment with specific ERalpha-agonist PPT and was inhibited by co-treatment with pure antiestrogen ICI 182,780 in MCF-7 and OVCAR-3 ovarian cancer cells. Treatment with cycloheximide totally inhibited estrogen effects suggesting that activation of icb-1 gene expression is no ERE-dependent early response but a secondary event requiring protein synthesis....Continue Reading

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Citations

Mar 25, 2009·Cancer Investigation·Anette SpringwaldOliver Treeck
May 9, 2012·Journal of Cellular Biochemistry·Oliver TreeckOlaf Ortmann
May 16, 2014·Journal of Ovarian Research·Susanne SchülerOliver Treeck
Mar 12, 2013·Cancer Letters·Oliver TreeckOlaf Ortmann
Mar 12, 2020·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Yasushi YamadaTanri Shiozawa

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