Sep 10, 2013

Expression of early growth response-1 in human gastric cancer and its relationship with tumor cell behaviors and prognosis

Pathology, Research and Practice
Eun MyungYoung-Eun Joo

Abstract

The early growth response-1 (Egr-1) is crucial in many cell regulatory processes related to the progression of human cancers. Its overexpression has been demonstrated in variable human cancers and may have prognostic significance. The aims of this current study were to evaluate whether Egr-1 affects invasive and oncogenic phenotypes of human gastric cancer cells, and to examine the relationships between its expression and various clinicopathological parameters, including survival in human gastric cancer patients. We investigated the biologic role of Egr-1 in tumor cell behavior by using a small interfering RNA in human gastric cancer cell lines, AGS and TMK1. The expression of Egr-1 by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry was investigated in human gastric cancer tissues. The knockdown of Egr-1 suppressed tumor cell migration and invasion in AGS and TMK1 cells. Egr-1 expression was significantly increased in human gastric cancer and metastatic lymph node tissues compared to the normal gastric mucosa and non-metastatic lymph node tissues. Positive expression of Egr-1 was significantly associated with tumor size, depth of invasion, lymph node metastasis, tumor stage an...Continue Reading

Mentioned in this Paper

Tumor Cells, Uncertain Whether Benign or Malignant
Prostatic Neoplasms
Gene Expression Regulation, Neoplastic
Tumor Suppressor Genes
Transfection
Tissue Membrane
Immunohistochemistry
TNM Staging System
Immunoreactivity
Formalin

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