Expression of endogenous proteins in maize hybrids in a multi-location field trial in India

Transgenic Research
Linga R GuthaShveta Bagga

Abstract

Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference or housekeeping proteins are expected to maintain a relatively stable expression pattern in healthy plants given their role in cellular functions. Understanding the effects of genotype, growth stage and location on the concentration of endogenous housekeeping proteins may provide insight into the contribution these factors could have on transgenic protein concentrations in GM crops. The concentrations of three endogenous proteins (actin, elongation factor 1-alpha, and glyceraldehyde 3-phosphate dehydrogenase) were measured in several different maize hybrids grown across multiple field locations over 2 years. Leaf samples were collected from healthy plants at three developmental stages across the growing seasons, and protein concentrations were quantified by indirect enzyme-linked immunosorbent assay (ELISA) for each protein. In general,...Continue Reading

References

Jan 5, 2000·Journal of Biotechnology·O ThellinE Heinen
Aug 20, 2004·BMC Plant Biology·Amy M BrunnerSteven H Strauss
Feb 20, 2008·Clinical Trials : Journal of the Society for Clinical Trials·Lelia DuleySalim Yusuf
Feb 24, 2009·BMC Molecular Biology·Anna R PaolacciMario Ciaffi
Jun 28, 2011·Journal of Experimental Botany·Xiaoming LiGuozhen Liu

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Methods Mentioned

BETA
transgenic
ELISA
electrophoresis

Software Mentioned

Image Quant
Softmax Gxp
SAS

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