Mar 1, 2003

Expression of endoplasmic reticulum molecular chaperon GRP94 in human lung cancer tissues and its clinical significance

Chinese Medical Journal
Qi WangShichang Yue

Abstract

To investigate the relationship between the expression of glucose regulated protein 94 (GRP94) at the level of mRNA and protein in vivo and in human lung cancer. RT-PCR, immunohistochemistry and/or Western blot were used in 54 cases of lung cancer tissues and corresponding normal lung tissues. There was a significant overexpression of GRP94 mRNA and protein in lung cancer tissues as compared with lung normal tissues. In lung cancer tissue, the relative level of GRP94 mRNA as evaluated by RT-PCR was 3.48 +/- 2.06, the level of GRP94 protein as evaluated by immunohistochemistry was + + to + + +, and by Western blot was 256.7 +/- 80.6. In lung normal tissue, the relative level of GRP94 mRNA was 2.01 +/- 1.83, the level of GRP94 protein was + to + + and 108.1 +/- 42.3. The differences in expression of GRP94 between the two tissues were significant (P < 0.05). Furthermore, the over-expression of GRP94 in the lung cancer tissues was correlated to grade of differentiation and stage of tumors. There was stronger expression in poor-differentiated tumors than in mild-to-high differentiated tumors (P < 0.05). There was also a stronger expression in stage III than in stage I and II tumors (P < 0.05). No statistically significant difference...Continue Reading

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Mentioned in this Paper

Heat-Shock Proteins 70
Immunohistochemistry
TNM Staging System
Lung
Western Blotting
Neoplasms
Immunocytochemistry
Carcinoma of Lung
Reverse Transcriptase Polymerase Chain Reaction
Cell Differentiation Process

About this Paper

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