PMID: 11331668May 2, 2001Paper

Expression of epithelial neutrophil-activating peptide 78 in cultured human endometrial stromal cells

Molecular Human Reproduction
K NasuI Miyakawa

Abstract

It has been demonstrated that human endometrial stromal cells (ESC) produce a variety of chemokines in vivo and in vitro. To evaluate the expression of epithelial neutrophil-activating peptide 78 (ENA-78) in the endometrium, concentrations of ENA-78 in cyclic endometrial tissues were measured using enzyme-linked immunosorbent assay. The expression of ENA-78 was also detected in cyclic endometrium by immunohistochemistry. Endometrial tissues in the secretory phase contained higher amounts of ENA-78 protein than did those in the proliferative phase. Immunofluorescence staining revealed that ENA-78 protein was localized mainly in the stroma of endometrium. In addition, to evaluate the involvement of inflammatory mediators and ovarian steroid hormones in the production of ENA-78 by ESC was evaluated by in-vitro studies. Unstimulated ESC constitutively secreted ENA-78. Progesterone, lipopolysaccharide, tumour necrosis factor-alpha, and interleukin-1beta significantly stimulated the expression of ENA-78 by ESC. It is suggested that the production of ENA-78 by ESC is regulated by progesterone as well as by the inflammatory mediators. The modulation of ENA-78 concentration in the local environment by these mediators may contribute to t...Continue Reading

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Citations

Jan 14, 2003·Fertility and Sterility·Shinichiro MineIsao Miyakawa
Mar 30, 2013·Human Reproduction Update·Fernando M ReisRobert N Taylor
Feb 22, 2011·Journal of Gastroenterology and Hepatology·Frank TackeBernd Schnabl
Jun 26, 2012·Cytokine & Growth Factor Reviews·Magali RancezRémi Cheynier
Apr 26, 2006·Transplant Immunology·Shosaku NomuraKunio Hayashi
Dec 4, 2014·Trends in Endocrinology and Metabolism : TEM·Brett D McKinnonMichael D Mueller
Dec 7, 2007·Fertility and Sterility·Sachiho NetsuMitsuaki Suzuki
Sep 15, 2005·Endocrine Reviews·Henry N JabbourHilary O D Critchley
Apr 8, 2003·The Journal of Clinical Endocrinology and Metabolism·Kotaro KitayaHideo Honjo

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