PMID: 8944608Nov 1, 1996Paper

Expression of epstein-barr virus encoded nuclear antigen 1 in benign and malignant tissues harbouring EBV

Journal of Clinical Pathology
J J OudejansC J Meijer

Abstract

To determine levels of expression of Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) in benign and malignant tissues harbouring EBV in relation to EBNA1 promoter usage. Expression of EBNA1 was investigated by means of immunohistochemistry using a mixture of two EBNA1 specific monoclonal antibodies, 1H4-1 and 2B4-1. The presence of EBV was detected by EBER1/2 RNA in situ hybridisation. Detection of promoter specific EBNA1 transcripts was by RT-PCR analysis. EBNA1 positive cells were detected in all 20 EBV associated B cell lymphomas, 18 of which had arisen in immunocompromised patients; in eight of nine EBV associated T cell lymphomas; in 11 of 27 EBV positive cases of Hodgkin's disease; and in reactive lymphoid tissue harbouring EBV, including four cases of infectious mononucleosis. A diffuse EBNA1 staining pattern was observed in most of the EBV associated B cell lymphomas and was comparable with the EBER1/2 staining pattern. In the T cell lymphomas the number of EBNA1 positive cells was usually considerably less than the number of EBER1/2 positive ones. RT-PCR analysis revealed that in tumours with restricted EBNA1 expression-that is, T cell lymphomas and Hodgkin's disease lesions, EBNA1 transcripts were usually generated ...Continue Reading

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Citations

Apr 25, 2000·Immunobiology·M S StaegeA B Reske-Kunz
Dec 17, 2002·Critical Reviews in Oncology/hematology·Jaap M MiddeldorpChris J L M Meijer
Feb 1, 1997·Critical Reviews in Oncology/hematology·J J OudejansC J Meijer
Mar 22, 2002·Nature Reviews. Immunology·D A Thorley-Lawson
Mar 27, 2004·The New England Journal of Medicine·David A Thorley-Lawson, Andrew Gross
Feb 8, 2008·Journal of Virology·Myung-Soo KangElliott Kieff
Mar 29, 2002·Journal of Clinical Pathology·P J van Diest, H Holzel
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·G J Babcock, D A Thorley-Lawson
Aug 9, 2005·The Journal of Allergy and Clinical Immunology·David A Thorley-Lawson
Sep 16, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anas Younes, Marshall E Kadin
Nov 17, 2004·Reviews in Medical Virology·Wolfgang Amon, Paul J Farrell
Jun 3, 2020·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Lucia MundoStefano Lazzi

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