PMID: 15248912Jul 14, 2004Paper

Expression of Epstein-Barr virus genes in EBV-associated gastric carcinoma

Ai zheng = Aizheng = Chinese journal of cancer
Yun WangBao-Hua Huang

Abstract

Epstein-Barr virus (EBV) is associated with the development of many malignant tumors. The forms of EBV and the expression of EBV genes in Burkitt's lymphoma and nasopharyngeal carcinoma (NPC) have been reported. These studies showed that the forms of EBV and the expression of EBV genes are various in different types of malignancies. However, there were only a few reports about the expression of EBV genes, especially the lytic genes, in gastric carcinoma tissues. This study was to determine the expression of EBV latent and lytic infection genes in gastric carcinoma at the transcriptional level by RT-PCR and Southern hybridization,and investigate the relationship between EBV-encoded genes and the tumorigenesis of gastric carcinoma at the molecular level. One hundred and eighty-five gastric carcinoma and corresponding para-carcinoma tissues were tested for EBV genome by polymerase chain reaction (PCR)-Southern analysis. EBV-encoded small RNA 1 (EBER1) of the PCR positive specimens was determined by in situ hybridization (ISH). Gastric carcinoma with positive EBER1 signals was confirmed EBV- associated gastric carcinoma (EBVaGC). RT-PCR and Southern hybridization were used to determine the expression of nuclear antigen (EBNA) promo...Continue Reading

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