PMID: 375025Mar 20, 1979Paper

Expression of Escherichia coli tryptophan operon in Rhizobium leguminosarum

Molecular & General Genetics : MGG
K NagahariK Sakaguchi

Abstract

RP4-trp hybrid plasmid containing Escherichia coli whole tryptophan operon was conjugatively transferred from E. coli to Rhizobium leguminosarum strains carrying mutations in different trp genes, converting their Trp- phenotype to Trp+. That the phenotype change of the R. leguminosarum cells was due to the presence of the E. coli tryptophan operon was verified by the isolation of RP4-trp hybrid plasmid from the R. leguminosarum conjugant cells, and by re-transfer of RP4-trp plasmid by conjugation back to E. coli trp and Pseudomonas putida trp strains. Enzymatic activities of anthranilate synthetase and beta subunit of tryptophan synthetase in crude extracts of R. leguminosarum cells containing RP4-trp plasmid were much higher than that of the wild-type cells and were not repressed by the presence of tryptophan in the culture medium.

References

Apr 21, 1977·Nature·K NagahariK Sakaguchi
Mar 1, 1978·Journal of Bacteriology·K Nagahari, K Sakaguchi
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A M ChakrabartyJ G Vacca
Jul 10, 1974·Biochemical and Biophysical Research Communications·R H Olsen, C Gonzalez
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S N CohenR B Helling
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J F MorrowR B Helling
Sep 1, 1974·Journal of General Microbiology·J E Beringer
Feb 1, 1973·Journal of Bacteriology·R H Olsen, P Shipley
Oct 1, 1971·Journal of Bacteriology·T Enatsu, I P Crawford
Dec 1, 1971·Journal of Bacteriology·N DattaM H Richmond

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