Expression of eukaryotic membrane proteins in eukaryotic and prokaryotic hosts.

Methods : a Companion to Methods in Enzymology
Athanasios KesidisAlice J Rothnie

Abstract

The production of membrane proteins of high purity and in satisfactory yields is crucial for biomedical research. Due to their involvement in various cellular processes, membrane proteins have increasingly become some of the most important drug targets in modern times. Therefore, their structural and functional characterization is a high priority. However, protein expression has always been more challenging for membrane proteins than for soluble proteins. In this review, we present four of the most commonly-used expression systems for eukaryotic membrane proteins. We describe the benefits and drawbacks of bacterial, yeast, insect and mammalian cells. In addition, we describe the different features (growth rate, yield, post-translational modifications) of each expression system, and how they are influenced by the construct design and modifications of the target gene. Cost-effective and fast-growing E. coli is mostly selected for the production of small, simple membrane proteins that, if possible, do not require post-translational modifications but has the potential for the production of bigger proteins as well. Yeast hosts are advantageous for larger and more complex proteins but for the most complex ones, insect or mammalian ce...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Oct 1, 1992·Current Opinion in Biotechnology·R Fleer
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Mar 1, 1989·Molecular and Cellular Biology·J M CreggC A Stillman
Oct 24, 1986·Nucleic Acids Research·P KerjanY Surdin-Kerjan
May 11, 1987·Nucleic Acids Research·J F TschoppT R Gingeras
Aug 1, 1984·Molecular and Cellular Biology·M Johnston, R W Davis
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H A de BoerM Vasser
Aug 1, 1995·Quarterly Reviews of Biophysics·R Grisshammer, C G Tate
Mar 15, 1995·Journal of the National Cancer Institute·D Holzman
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·D A Siegele, J C Hu
Nov 14, 1997·Immunotechnology : an International Journal of Immunological Engineering·M A ClarkR L Ward
Oct 6, 1999·Current Opinion in Biotechnology·F Baneyx
May 29, 2000·The Journal of Biological Chemistry·C LegrosO Pongs
Jun 23, 2000·Enzyme and Microbial Technology· van Dijk R van der Klei I
Mar 17, 2001·Molecular Biotechnology·S H Bass, D G Yansura
Jul 5, 2001·Biochemical and Biophysical Research Communications·C OzvegyB Sarkadi
Jan 11, 2002·European Journal of Biochemistry·H Markus Weiss, Reinhard Grisshammer
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Philip J ReevesH Gobind Khorana
Apr 2, 2003·Journal of Biotechnology·Geen Dong -hangHungwen Chen
May 6, 2003·Biochimica Et Biophysica Acta·A G Lee
Sep 10, 2003·Protein Expression and Purification·Richard J JennyRoger L Lundblad
Feb 3, 2004·Biotechnology and Bioengineering·Hubertus HohenblumDiethard Mattanovich
Feb 7, 2004·The Journal of Biological Chemistry·Natalie M Stano, Smita S Patel
Jul 13, 2004·Trends in Biotechnology·Claes GustafssonJeremy Minshull
Nov 9, 2004·Current Opinion in Cell Biology·Rammohan V Rao, Dale E Bredesen
Dec 21, 2004·Journal of Biotechnology·Hans Peter Sørensen, Kim Kusk Mortensen
Jul 9, 2005·Science·Stephen B LongRoderick Mackinnon
Aug 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Marie JidenkoChristine Jaxel

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Citations

May 22, 2021·Biochimica Et Biophysica Acta. Biomembranes·Roslyn M Bill, Kristina Hedfalk
Aug 1, 2020·Methods : a Companion to Methods in Enzymology·Anass Jawhari
Oct 9, 2021·Emerging Topics in Life Sciences·James Birch, Andrew Quigley

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