Expression of exogenous Sam68, the 68-kilodalton SRC-associated protein in mitosis, is able to alleviate impaired Rev function in astrocytes

Journal of Virology
Jinliang LiJohnny J He

Abstract

Human immunodeficiency virus type 1 (HIV-1) gene expression in astrocytes is restricted, resulting in a brief and limited synthesis of HIV-1 viral structural proteins. Impaired Rev function has been documented in these cells. However, the molecular mechanisms underlying the impaired Rev function are not fully understood. Using the astroglial cell line U87.MG as a model, we report here that HIV-1 gene expression down-regulated expression of Sam68, the 68-kDa Src-associated protein in mitosis, which was constitutively expressed at a lower level in astrocytes. Elevating the endogenous level of Sam68 expression considerably restored HIV-1 Rev function in astrocytes, as determined by a Rev-dependent reporter gene assay. However, elevation of Sam68 expression achieved only a modest increase in HIV-1 production, further supporting the notion that there are multiple cellular restrictions of HIV-1 gene expression in astrocytes. Mutagenesis analysis identified the region between amino acids 321 and 410 of Sam68 as being directly involved in the binding of Sam68 to Rev, while a double mutation in Rev, L78D and E79L, like those in the dominant-negative Rev mutant M10, eliminated Rev binding to Sam68. Moreover, subcellular fractionation and...Continue Reading

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Citations

Mar 2, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jinfeng LiuJohnny J He
Oct 16, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Lena Al-Harthi
Dec 4, 2003·Biochimica Et Biophysica Acta·Kiven E Lukong, Stéphane Richard
Dec 4, 2003·Experimental Cell Research·Séverine DemartRuth Brack-Werner
Jul 2, 2003·Nature Cell Biology·Yong-Hui ZhengB Matija Peterlin
Jul 9, 2010·The Journal of Biological Chemistry·N Helge MeyerMichael Sattler
Feb 11, 2005·Nucleic Acids Research·Suhasini ModemThipparthi R Reddy
Aug 7, 2009·BMC Complementary and Alternative Medicine·In-Woo ParkJohnny J He
Jun 23, 2004·RNA·Meredith McLarenAlan Cochrane
Aug 2, 2002·Expert Opinion on Investigational Drugs·Roger G Ptak
Jun 17, 2011·Journal of Cellular Physiology·Suhasini ModemThipparthi R Reddy
Nov 3, 2004·Molecular and Cellular Neurosciences·Betty Y ZhouJohnny J He
Jul 19, 2005·Gene·John A DangerfieldWalter H Günzburg
Mar 27, 2009·The Journal of General Virology·H C T GroomA M L Lever
Jul 21, 2009·Experimental Cell Research·Jorge Henao-Mejia, Johnny J He
Nov 30, 2004·Virology·Jianhua FangRoger J Pomerantz
Dec 19, 2008·Virology·Meredith McLaren, Alan Cochrane
Jun 24, 2006·Journal of Cellular Biochemistry·Kameswara R BadriThipparthi R Reddy
Mar 24, 2006·Journal of Neuroscience Research·Jessica GardnerAnuja Ghorpade
Sep 24, 2005·The Journal of Biological Chemistry·Kiven Erique LukongStéphane Richard
Dec 20, 2014·Journal of Neurovirology·Xiaoyu Luo, Johnny J He
Aug 2, 2017·Journal of Neurovirology·Pia Pužar DominkušMetka Lenassi

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