Expression of extracellular matrix-remodeling proteins is altered in vaginal tissue of premenopausal women with severe pelvic organ prolapse

Reproductive Sciences
May AlarabOksana Shynlova

Abstract

The molecular etiology of pelvic organ prolapse (POP) is complex and not well understood. We compared the expression/activity of extracellular matrix (ECM)-processing (procollagen I N-proteinase/ a disintegrin and metalloproteinase with thrombospondin motifs [ADAMTS]-2,-3,-14) and ECM-degrading (matrix metalloproteinase [MMP]-1, -2, -7, -8, -9, -12) enzymes and their natural tissue inhibitors (tissue inhibitors of metalloproteinase [TIMP]-1,-2,-3,-4) in vaginal tissues from premenopausal women with advanced POP (POP-Q stage ≥ 3) and asymptomatic controls (POP-Q = 0). We sampled the anterior vaginal wall of 36 premenopausal women (17 patients with POP and 19 controls) undergoing total hysterectomy. Exclusion criteria include steroid therapy, malignancy, previous pelvic surgery, and connective tissue diseases. Total RNAs and proteins were quantified by real-time polymerase chain reaction, immunoblotting, and Luminex assay; MMPs activity was analyzed by zymography and tissue localization by immunohistochemistry. The MMP-2 gelatinase activity as well as expression of 58-kDa isoform of ADAMTS-2 was upregulated in patients with POP, irrespective of menstrual phase status, secretory or proliferative, when compared to controls (P < .05...Continue Reading

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Citations

Feb 4, 2016·Neurourology and Urodynamics·Mahshid VashaghianTheo H Smit
Nov 1, 2016·Tissue Engineering. Part a·Mahshid VashaghianTheodoor Henri Smit
May 9, 2018·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Antonella VetuschiRoberta Sferra
Sep 17, 2016·Female Pelvic Medicine & Reconstructive Surgery·Ali BorazjaniMargot S Damaser
Jul 9, 2020·International Journal of Molecular Sciences·Alejandra M Ruiz-ZapataEgbert Oosterwijk

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Methods Mentioned

BETA
hysterectomy
dissection
biopsies
biopsy
PCR
Reverse
immunoprecipitation assay
Protein Assay
electrophoresis

Software Mentioned

Bio
Primer Express
Image J
Prism
Plex Manager
BLAST

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