Expression of FAM20C in the osteogenesis and odontogenesis of mouse.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Xiaofang WangC Qin

Abstract

Mutations in FAM20C were recently identified as the cause of lethal osteosclerotic bone dysplasia, which highlighted the important role of this molecule in biomineralization. No systematic studies have been performed to evaluate the expression pattern of this relatively new molecule in the developmental processes of bone and tooth. In the present study, we analyzed in detail the expression profile of FAM20C during osteogenesis and odontogenesis using ISH and IHC approaches. The specimens analyzed were mouse tissues spanning embryonic day 13.5 (E13.5) to postnatal 8 weeks. The earliest presence of FAM20C was observed at E14.5. During osteogenesis, FAM20C mRNA was detected in the chondrocytes and osteoblasts of the long bone, whereas its protein was observed in the extracellular matrix (ECM) of bone and in the cytoplasm of the chondrocytes, osteoblasts, and osteocytes. During odontogenesis, FAM20C mRNA was detected in the ameloblasts, odontoblasts, cementoblasts, and periodontal ligament fibroblasts, whereas its protein was observed in the matrices of dentin, enamel, and alveolar bone and in the cytoplasm of the aforementioned cells. The temporospatial expression profile revealed in this study indicates that FAM20C is an ECM prot...Continue Reading

References

Aug 26, 1998·American Journal of Medical Genetics·A Rejjal
Apr 26, 2001·Developmental Biology·M SwartzC E Krull
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·W BiB de Crombrugghe
Oct 9, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jian Q FenMary MacDougall
Nov 10, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Otto BabaWilliam T Butler
Oct 22, 2005·Orthodontics & Craniofacial Research·C M Giachelli
Mar 21, 2007·The Journal of Biological Chemistry·Jianjun HaoAnne George
Nov 27, 2007·Journal of Dental Research·D C WescottM C Meikle
Mar 3, 2009·Clinical Genetics·M A SimpsonA H Crosby

❮ Previous
Next ❯

Citations

Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Vincent S TagliabracciJack E Dixon
Jul 16, 2014·European Journal of Medical Genetics·Víctor FaundesBarry Chioza
May 31, 2014·Connective Tissue Research·Xiaofang WangChunlin Qin
Jan 2, 2013·Trends in Biochemical Sciences·Vincent S TagliabracciJack E Dixon
Sep 10, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Iris LindbergKevin Z Li
Dec 30, 2014·International Journal of Oral Science·Er-Xia DuJian-Jun Hao
Aug 15, 2014·Stem Cells and Development·Dong-Soo ParkChang-Sung Kim
Sep 27, 2016·European Journal of Medical Genetics·Siham Chafai ElalaouiAbdelaziz Sefiani
Dec 18, 2014·Journal of Dental Research·X WangC Qin
Mar 17, 2017·Journal of Human Genetics·Piranit Nik KantaputraNipon Chaisrisookumporn
Sep 13, 2013·Journal of Dental Research·X WangC Qin
Mar 30, 2018·International Journal of Oral Science·Wen-Juan YanXiao-Fang Wang
Jun 14, 2016·Scientific Reports·Yoshio OhyamaYoshiyuki Mochida
Sep 1, 2012·The Journal of Biological Chemistry·Xiaofang WangChunlin Qin
Sep 22, 2019·The Journal of Cell Biology·Adina Gerson-GurwitzArshad Desai
Nov 2, 2019·Journal of Molecular Histology·Lili LiYongbo Lu
Apr 28, 2020·Calcified Tissue International·Mehmet EltanSerap Turan
Sep 2, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiudong YangXiaofang Wang
Mar 14, 2019·International Journal of Molecular Medicine·Nan MiaoBin Zhang
Oct 15, 2020·Scientific Reports·Ju-Hsien LinYoshiyuki Mochida
Dec 18, 2020·Frontiers in Genetics·Jian Ming Khor, Charles A Ettensohn
Mar 30, 2020·Gene Expression Patterns : GEP·Jingyi WuXiaofang Wang
Mar 25, 2021·The Journal of Biological Chemistry·Carolyn A WorbySourav Banerjee
Dec 25, 2012·Journal of Cell Science·Leila MohazabHannu Larjava
Apr 28, 2021·European Journal of Oral Sciences·Hua ZhangChunlin Qin
Feb 27, 2020·Cells, Tissues, Organs·Hua ZhangChunlin Qin

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA
transgenic
transfection

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.