PMID: 9177233Jun 10, 1997Paper

Expression of Fas ligand in liver metastases of human colonic adenocarcinomas

Proceedings of the National Academy of Sciences of the United States of America
K ShirakiH Takahashi

Abstract

Fas ligand (FasL) plays a pivotal role in lymphocyte cytotoxicity and the maintenance of immunological homeostasis. Since FasL has been implicated in the existence of immunologically privileged body sites by inducing apoptosis of activated T lymphocytes, we investigated the expression of FasL in human colon cancers. We found that two out of seven primary tumors and all four hepatic metastatic tumors of surgically obtained colonic adenocarcinoma expressed FasL mRNA and protein, detected by reverse transcription-coupled PCR and by immunohistochemical staining, respectively. Expression of FasL was not detected in normal colonic epithelial cells. FasL mRNA was also expressed in some human colonic adenocarcinoma cell lines including SW480, SW1116, and LS180 cells. Cell-surface-associated FasL was detected in these human colon cancer cells by fluorescence immunocytochemical staining. In addition, the expressed FasL was demonstrated to be functional, since coculture experiments using FasL-expressing SW480 cells resulted in apoptosis of Jurkat T leukemia cells that are sensitive to Fas-mediated apoptosis, and this process was specifically inhibited by the neutralizing anti-human FasL antibody. Thus, our findings and other data suggest ...Continue Reading

References

Jul 1, 1992·Diseases of the Colon and Rectum·Y KubotaT Muto
Nov 1, 1989·Cytometry·I J Fidler
Jan 1, 1985·Critical Reviews in Food Science and Nutrition·A AsgharT Yasui
Aug 1, 1983·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·E N Spremulli, D L Dexter
Nov 17, 1995·Science·J W Streilein
Nov 17, 1995·Science·T S GriffithT A Ferguson
Dec 1, 1994·Experimental Cell Research·R NiS Nagata
Jan 1, 1995·The Journal of Experimental Medicine·M R AldersonD H Lynch
Feb 2, 1995·Nature·J DheinP H Krammer
Mar 10, 1995·Science·S Nagata, P Golstein
Jan 1, 1995·Immunology Today·S Nagata, T Suda
Apr 7, 1995·Cell·G Berke
Aug 1, 1995·Experimental Cell Research·M WeisS C Chow
Oct 19, 1995·Nature·D BellgrauR C Duke
Nov 1, 1995·The Journal of Experimental Medicine·P R GalleL Runkel
Aug 26, 1993·Nature·J OgasawaraS Nagata
Nov 19, 1993·Cell·P C Doherty
May 19, 1995·Cell·J L Cleveland, J N Ihle
Oct 1, 1994·International Immunology·T TakahashiS Nagata
Oct 28, 1994·Biochemical and Biophysical Research Communications·E MitaT Kamada
Jul 1, 1994·Gut·A K NigamP B Boulos
Dec 1, 1995·Immunology Today·D H LynchM R Alderson
Mar 1, 1996·Nature Medicine·M TanakaS Nagata
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·T H KuoR M Hoffman
Nov 22, 1996·Science·N Williams
Feb 1, 1997·Nature Medicine·K SeinoH Yagita
Feb 7, 1997·Cell·S Nagata
Sep 1, 1996·The Journal of Experimental Medicine·J O'ConnellF Shanahan

❮ Previous
Next ❯

Citations

Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D VermijlenE Wisse
Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T Patel
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Alessandro StrebelPeter Erb
Dec 10, 2002·International Journal of Cancer. Journal International Du Cancer·Deirdre ToomeyDavid Bouchier-Hayes
Jan 17, 2002·The Journal of Pathology·Tineke TimmerSteven de Jong
Apr 16, 1998·Springer Seminars in Immunopathology·J AllisonH Yagita
Oct 13, 1998·Cancer Immunology, Immunotherapy : CII·D B Chappell, N P Restifo
Sep 5, 2002·Biochemical and Biophysical Research Communications·Fumika OriiYutaka Kohgo
Sep 5, 2002·Mathematical Biosciences·Steven D WebbReginald G Fish
Mar 19, 1999·Immunology Today·J O'ConnellF Shanahan
Feb 13, 2001·Vaccine·P Moingeon
Oct 31, 1998·Current Opinion in Immunology·P R WalkerP Y Dietrich
Oct 18, 2003·Seminars in Immunology·Min Li-Weber, Peter H Krammer
Jun 6, 2003·Cytokine & Growth Factor Reviews·Hae-ock Lee, Thomas A Ferguson
Apr 18, 2001·Cell Research·X Q Fan, Y J Guo
Mar 29, 2002·British Journal of Haematology·Helmut R SalihVolkmar Nuessler
Mar 16, 2002·Journal of Gastroenterology and Hepatology·Tae-Bum LeeCheol-Hee Choi
Aug 31, 1999·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·M W BennettF Shanahan
Sep 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T ShimonishiY Nakanuma
Feb 18, 1999·Human Gene Therapy·F Paillard
Feb 18, 1999·Human Gene Therapy·R P McGuinnessJ G McArthur
Oct 28, 2008·Nucleic Acids Research·Anne DallasBrian H Johnston
Mar 14, 2000·Annals of Surgery·M KornmannM Korc
Jan 16, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·S H LeeN J Yoo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis