Expression of fibroblast growth factor-1 (FGF-1), FGF-2 and FGF receptor-1 in a human salivary-gland adenocarcinoma cell line: evidence of growth

International Journal of Cancer. Journal International Du Cancer
Y MyokenK Takada

Abstract

Fibroblast growth factor-1 (FGF-1) and FGF-2 are heparin-binding polype ptides which express potent mitogenic properties in neoplastic cells. In the present study, we have examined the contribution of endogenous FGF-1 and FGF-2 to the autocrine growth of HSY human salivary-gland adenocarcinoma cells in vitro. Using specific monoclonal antibodies against FGF-1 and FGF-2, immunohistochemical analysis of HSY cells revealed strong expression of both FGF-1 and FGF-2 in the cytoplasm and nucleus. Consistent with these data, 2 molecular mass species of FGF-1 (16 and 18 kDa) and 3 FGF-2 (18, 24 and 27 kDa) were identified in HSY cells by Western-blot analysis. Scatchard analysis of FGF binding sites on HSY cells indicated the presence of 23,000 [125I]FGF-1 binding sites/cells with a dissociation constant (KD) of 178 pM and 13,000 [125I]FGF-2 binding sites/cell with a KD of 102 pM. In addition, HSY cells were shown to express the mRNA for FGF receptor-1 (FGFR-1) by reverse transcription-polymerase chain reaction (RT-PCR), confirming the existence of high-affinity FGF binding sites. The influence of endogenous FGF-1 and FGF-2 on HSY cell growth was evaluated by suppressing the expression and activity of FGF by using anti-sense oligonucle...Continue Reading

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Citations

Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangT Okamoto
Feb 4, 2010·Journal of Medicinal Chemistry·Krishna P RavindranathanWilliam L Jorgensen
Jan 1, 1997·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Y MyokenK Takada
Apr 22, 1999·The Journal of Pathology·J JacquemierD Birnbaum
Mar 27, 2004·Journal of Dental Research·S KapasJ P Hinson
Feb 3, 2004·In Vitro Cellular & Developmental Biology. Animal·Nobuyuki AsadaTetsuji Okamoto
Dec 29, 2011·Journal of Cancer Research and Clinical Oncology·C G StoehrB Walter
Oct 14, 2009·International Journal of Immunopathology and Pharmacology·D MarzioniR Montironi
Nov 30, 2011·Breast Cancer Research and Treatment·Juan P CerlianiCaroline A Lamb

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