PMID: 9420196Jan 7, 1998Paper

Expression of foreign proteins by poliovirus polyprotein fusion: analysis of genetic stability reveals rapid deletions and formation of cardioviruslike open reading frames

Journal of Virology
S Mueller, E Wimmer

Abstract

Using a strategy developed by R. Andino, D. Silvera, S. D. Suggett, P. I. Achacoso, C. J. Miller, D. Baltimore, and M. B. Feinberg (Science 265:1448-1451, 1994), we constructed recombinant polioviruses by fusing the open reading frame (ORF) of the green fluorescent protein gene (gfp) of Aequorea victoria or the gag gene (encoding p17-p24) of human immunodeficiency virus type 1 (HIV-1) to the N terminus of the poliovirus polyprotein. All poliovirus expression vectors constructed by us and those obtained from Andino et al. were found to be severely impaired in viral replication and genetically unstable. Upon replication, inserted sequences were rapidly deleted as early as the first growth cycle in HeLa cells. However, the vector viruses did not readily revert to the wild-type sequence but rather retained some of the insert plus the artificial 3Cpro/3CDpro cleavage site, engineered between the heterologous sequence and the poliovirus polyprotein, to give rise to genotypes reminiscent of cardioviruses. These virus variants that carry a small leader polypeptide were now relatively stable, and they grew better than their progenitor strains. Reverse transcription followed by PCR and sequence analysis of the genomic RNAs reproducibly r...Continue Reading

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Citations

Apr 25, 2000·Journal of Virology·K HöflingJ Smith Leser
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Taeg S Kim, Stanley Perlman
May 29, 2004·The Journal of General Virology·Magdalena T TostesonMarie Chow
Dec 5, 2008·Expert Review of Vaccines·Andreas HenkePeter Wutzler
Nov 27, 2009·Journal of Virology·Natalya L TeterinaEllie Ehrenfeld
Dec 14, 2011·Journal of Virology·Yutong SongEckard Wimmer
Apr 6, 2013·The Journal of General Virology·Julian SeagoTerry Jackson
Jul 10, 2016·The Journal of General Virology·Huibin YuChangjiang Weng
Jan 29, 2020·Nature Communications·Mubeen M MosahebMatthias Gromeier
Aug 7, 2002·Journal of Virology·Andrew T DufresneMatthias Gromeier
Dec 13, 2003·Journal of Virology·Pranav Danthi, Marie Chow
Mar 16, 2018·Microbiology and Molecular Biology Reviews : MMBR·Vadim I Agol, Anatoly P Gmyl
Nov 3, 2017·Journal of Virology·Tomokazu TamuraYoshiharu Matsuura
Aug 19, 2018·Influenza and Other Respiratory Viruses·Mingyuan HanMarc B Hershenson

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