Expression of free radicals by peritoneal cells of sheep during the early stages of Fasciola hepatica infection

Parasites & Vectors
R Pérez-CaballeroAlvaro Martínez-Moreno

Abstract

The majority of vaccination studies against infection with F. hepatica in a natural host have been conducted at the late stage of the infection when the host's immune response is already immunomodulated by the parasite towards a Th2 non-protective response. This study was aimed at analysing the dynamic of the cell populations present in peritoneal liquid and the production of free radicals by the peritoneal leukocytes in infected and vaccinated sheep with recombinant cathepsin L1 of F. hepatica (rFhCL1) in early stages of the infection. Forty-five sheep were divided into three groups: Group 1 remained as negative control (n = 5), Group 2 (n = 20) was challenged with F. hepatica and Group 3 (n = 20) was vaccinated with rFhCL1 and challenged with F. hepatica. After the slaughtering, peritoneal lavages were carried out at 1, 3, 9 and 18 days post-infection (dpi) to isolate peritoneal cell populations. Flow cytometry was conducted to assess levels of hydrogen peroxide (H2O2) and nitric oxide (NO). There was a significant increase in the total number of leukocytes at 9 and 18 dpi in infected and vaccinated groups. Production of H2O2 was significantly increased in peritoneal granulocytes in both infected and vaccinated groups. Produc...Continue Reading

References

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Methods Mentioned

BETA
electrophoresis
flow cytometry
lavage
ELISA
FCS

Related Concepts

Cathepsin L1, Fasciola hepatica
Metazoa
Helminth Antibodies
Cathepsins
Fasciola hepatica
Fascioliasis
Free Radicals
Perhydrol
Leukocytes
Nitric Oxide

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