Expression of freezing and fear-potentiated startle during sustained fear in mice

Genes, Brain, and Behavior
T DaldrupT Seidenbecher

Abstract

Fear-potentiated acoustic startle paradigms have been used to investigate phasic and sustained components of conditioned fear in rats and humans. This study describes a novel training protocol to assess phasic and sustained fear in freely behaving C57BL/6J mice, using freezing and/or fear-potentiated startle as measures of fear, thereby, if needed, allowing in vivo application of various techniques, such as optogenetics, electrophysiology and pharmacological intervention, in freely behaving animals. An auditory Pavlovian fear conditioning paradigm, with pseudo-randomized conditioned-unconditioned stimulus presentations at various durations, is combined with repetitive brief auditory white noise burst presentations during fear memory retrieval 24 h after fear conditioning. Major findings are that (1) a motion sensitive platform built on mechano-electrical transducers enables measurement of startle responses in freely behaving mice, (2) absence or presence of startle stimuli during retrieval as well as unpredictability of a given threat determine phasic and sustained fear response profiles and (3) both freezing and startle responses indicate phasic and sustained components of behavioral fear, with sustained freezing reflecting un...Continue Reading

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Citations

Feb 16, 2016·Behavioural Brain Research·Thomas SeidenbecherHans-Christian Pape
Apr 4, 2016·Neurobiology of Learning and Memory·Thiemo DaldrupHans-Christian Pape
Jun 18, 2016·BioMed Research International·Jacek PolechońskiAgnieszka Nawrocka
Aug 18, 2017·Learning & Memory·Travis D Goode, Stephen Maren
Aug 28, 2020·Frontiers in Molecular Neuroscience·Natascha SchaeferCarmen Villmann
Oct 1, 2017·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Jacek PolechońskiJanusz Błaszczyk
May 11, 2018·Brain Structure & Function·Manuela CerinaThomas Budde
Oct 15, 2019·Frontiers in Molecular Neuroscience·Marziah HakimFatemeh Chehrehasa
Jun 14, 2020·Journal of Neuroinflammation·Manuela CerinaSven G Meuth
Jan 16, 2021·Social Cognitive and Affective Neuroscience·Milena RadomanStephanie M Gorka

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