PMID: 11337196May 5, 2001Paper

Expression of fructose-1,6-bisphosphatase mRNA isoforms in normal and basal forebrain cholinergic lesioned rat brain

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
T LöfflerR Schliebs

Abstract

Fructose-1,6-bisphosphatase is one of the key enzymes in the gluconeogenic pathway predominantly occurring in liver, kidney and muscle. In the brain, fructose-1,6-bisphosphatase has been suggested to be an astrocyte-specific enzyme but the functional importance of glyconeogenesis in the brain is still unclear. To further elucidate the cellular source of fructose-1,6-bisphosphatase in the brain, non-radioactive in situ hybridizations were performed using digoxigenin-labeled RNA probes based on the sequence of recently cloned rat liver and muscle fructose-1,6-bisphosphatase cDNAs. In situ hybridization using a riboprobe for the liver isoform revealed a location of the hybridization signal mainly in neurons, while rat muscle fructose-1,6-bisphosphatase mRNA was detected in both neurons and astrocytes in the hippocampal formation and in layer I of the cerebral cortex.RT-PCR using RNA preparations of rat astrocytes, neurons, and adult whole brain demonstrated a localization of liver fructose-1,6-bisphosphatase mRNA isoform in neurons but not in astrocytes. The muscle fructose-1,6-bisphosphatase mRNA isoform could be detected by RT-PCR in total rat brain, astrocytic, and neuronal mRNA preparations. The isoforms of fructose-1,6-bispho...Continue Reading

References

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Citations

May 5, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R Schliebs
Mar 20, 2014·Metabolic Brain Disease·Mauro DiNuzzoFederico Giove
Jul 25, 2009·Proteins·Agnieszka GizakDariusz Rakus
Aug 6, 2010·Microscopy Research and Technique·Z D VelásquezP Gómez-Ramos
Apr 7, 2016·Acta Crystallographica. Section D, Structural Biology·Jakub BarciszewskiAndrzej Dzugaj

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