PMID: 2496202May 1, 1989Paper

Expression of functional human monoamine oxidase A and B cDNAs in mammalian cells

Journal of Neurochemistry
N C LanJ C Shih

Abstract

Monoamine oxidase (MAO) A and B are important enzymes that metabolize biogenic amines throughout the body. Previous studies had suggested that both MAO A and B consist of two subunits of molecular masses of 63 and 60 kilodaltons, respectively. The cDNAs encoding one subunit of human liver MAO A and B have been expressed in mammalian cells by transfection of the individual clones. The proteins expressed from these cDNAs are shown to be catalytically active. Similar to the endogenous enzymes, the expressed MAO A prefers serotonin as a substrate and is sensitive to the inhibitor clorgyline. In contrast, the expressed MAO B prefers phenylethylamine as a substrate and is sensitive to the inhibitor deprenyl. These results suggest that a single polypeptide of MAO A (or B), existing as either a monomer or homodimer, is enzymatically active. The ability to obtain functional MAO A and B from their respective cDNA clones allows us to study further the structure and function relationships of these important enzymes.

References

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Citations

Jan 1, 1992·Psychopharmacology·S W KwanC W Abell
Jan 1, 1990·Pharmacology & Therapeutics·W WeylerX O Breakefield
Jun 5, 1992·European Journal of Pharmacology·M GerlachM B Youdim
Mar 14, 2001·European Journal of Pharmacology·G BompartA Parini
Sep 18, 2003·Bioorganic & Medicinal Chemistry·Xingliang LuRichard B Silverman
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J GrimsbyJ C Shih
Feb 1, 1991·The Journal of Pharmacy and Pharmacology·M D BalsaM Unzeta
Apr 15, 1999·Annual Review of Neuroscience·J C ShihM J Ridd
Jun 23, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marco BortolatoJean C Shih
Jan 18, 2008·Neuroscience and Biobehavioral Reviews·Flavio KapczinskiRobert M Post
Jan 19, 2007·The European Journal of Neuroscience·Karine GuillemLuis Stinus
Dec 31, 1990·Biochemical and Biophysical Research Communications·W WeylerJ I Salach
Dec 30, 2014·Bioorganic & Medicinal Chemistry·Hanno SchiefersteinFrank Rösch
Oct 1, 1990·Journal of Neurochemistry·J GrimsbyJ C Shih
Jul 20, 2017·The Journal of Pathology·Pei Chuan LiJean Chen Shih
Jul 22, 1998·Proteins·J Wouters, G Baudoux
Sep 12, 2000·The Annals of Pharmacotherapy·L M RoseV F Mauro
Jun 7, 2007·Neurochemical Research·Jean Chen Shih
Aug 18, 2020·BMC Complementary Medicine and Therapies·Pei-Chuan LiJean Chen Shih
May 24, 2001·The Journal of Biological Chemistry·I RebrinJ C Shih
Apr 11, 2020·Scientific Reports·Hui Min LeeLee Fah Yap
Dec 31, 1991·Biochemical and Biophysical Research Communications·A K TanT P Singer

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