PMID: 9641526Jun 26, 1998Paper

Expression of GABA(A) receptors by reactive astrocytes in explant and primary cultures of rat CNS

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
E HösliL Hösli

Abstract

The presence of GABA(A)-receptors on astrocytes was studied in explant and primary cultures of rat cerebellum, hippocampus and spinal cord by means of immunohistochemistry. For these studies we have used the monoclonal antibody bd 17 against the beta2- and beta3-subunits of GABA(A)-receptor. In explant cultures many neurones were intensely stained with the GABA(A)-receptor antibody whereas adjacent astrocytes revealed little or no immunoreactivity. In the far outgrowth zone of explant culture, however, many immunostained astrocytes were observed. In primary astrocyte cultures, only a few cells were stained by the antibody. Astrocytes which became reactive after producing an artificial scar or after addition of certain compounds such as dibutyryl cyclic AMP, interleukin-6, basic fibroblast growth factor and kainic acid, also revealed GABA(A)-receptor immunoreactivity. Furthermore, these astrocytes were intensely stained for glial fibrillary acidic protein and vimentin. From our studies we conclude that only a sub-population of normal astrocytes are immunopositive for the GABA(A)-receptor antibody whereas astrocytes which become reactive following injury of the tissue or after addition of dibutyryl cyclic AMP, the cytokine interl...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P BovolinD Grayson
Nov 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C K PetitoM L Lesser
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Bormann, H Kettenmann
Aug 1, 1988·Journal of Neurocytology·M B GraeberG W Kreutzberg
Feb 1, 1985·The Journal of Cell Biology·M E Hatten
Nov 23, 1984·Neuroscience Letters·H KettenmannM Schachner
Jul 2, 1981·Neuroscience Letters·L HösliH Landolt
Jul 1, 1983·Journal of Neurochemistry·J T Coyle
Jan 1, 1984·Journal of Neuroscience Research·G Fischer
Nov 1, 1995·Journal of Neurochemistry·M BureauT Grisar
Apr 1, 1993·Progress in Neurobiology·E Hösli, L Hösli
May 1, 1994·Journal of Neuropathology and Experimental Neurology·M D Norenberg
Dec 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E Hösli, L Hösli
Jun 1, 1996·Progress in Neurobiology·A Vernadakis
Feb 1, 1997·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E Hösli, L Hösli
Aug 1, 1997·Progress in Neurobiology·R A Gadient, U H Otten

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Citations

Mar 10, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E HösliL Hösli
Sep 10, 2003·Domestic Animal Endocrinology·M GalbiatiR C Melcangi
Feb 3, 2012·Physiological Reviews·Hanns Ulrich ZeilhoferGonzalo E Yévenes
Jul 25, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A SimiN Tindberg
May 24, 2019·Scientific Reports·Samah Kalakh, Abdeslam Mouihate

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