Expression of gamma-aminobutyric acid and glutamic acid decarboxylases in rat descending colon and their relation to epithelial differentiation

Chinese Journal of Digestive Diseases
Fang Yu WangMasahito Watanabe

Abstract

To detect the expression of gamma-aminobutyric acid (GABA) and glutamic acid decarboxylases (GADs; including two isoforms GAD65 and GAD67) in the epithelial growth zones of the descending colon in rats, and to investigate their relation to epithelial differentiation and proliferation. The expression of GABA and GADs in rat descending colon was investigated by immunofluorescent staining and confocal laser scanning techniques, and goblet cells were further investigated by wheat-germ agglutinin histochemistry. In addition, GAD65 and GAD67 mRNAs were also detected by reverse transcription-polymerase chain reaction. Furthermore, evaluation of cell kinetics in colonic epithelia was conducted by ABC immunostaining using a monoclonal antibody against proliferating cell nuclear antigen (PCNA). Immunoreactive GABA and GADs were distributed in the upper third of the crypts and at the luminal surface in the rat descending colon. Strong staining for GABA and GADs was localized mainly in the cytoplasm of epithelial cells near the neck of the crypts and along the luminal surface. In addition, GABA and GAD65 were also detected at the lamina propria in colonic mucosa. No staining for GABA or GADs was found in goblet cells. GAD65 and GAD67 mRNAs...Continue Reading

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Citations

Sep 27, 2006·Pituitary·Joseph E Ippolito
Mar 16, 2013·Human Molecular Genetics·Swagatika Sahoo, Ines Thiele
Oct 17, 2015·Genes & Nutrition·Hannah-Sophie BraunCarolin Deiner
Oct 31, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Andrea KwakowskyGábor Szabó
May 12, 2019·The Journal of Physiology·Emanuel Loeza-AlcocerMichael S Gold

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