Expression of GDNF and GFR alpha 1 in mouse taste bud cells

The Journal of Comparative Neurology
Masako TakedaKentaro Kawakoshi

Abstract

GDNF (glial cell line-derived neurotrophic factor) affects the survival and maintenance of central and peripheral neurons. Using an immunocytochemical method, we examined whether the taste bud cells in the circumvallate papillae of normal mice expressed GDNF and its GFR alpha 1 receptor. Using double immunostaining for either of them and NCAM, PGP 9.5, or alpha-gustducin, we additionally sought to determine what type of taste bud cells expressed GDNF or GFR alpha 1, because NCAM is reported to be expressed in type-III cells, PGP 9.5, in type-III and some type-II cells, and alpha-gustducin, in some type-II cells. Normal taste bud cells expressed both GDNF and GFR alpha 1. The percentage of GDNF-immunoreactive cells among all taste bud cells was 31.63%, and that of GFR alpha 1-immunoreactive cells, 83.21%. Confocal laser scanning microscopic observations after double immunostaining showed that almost none of the GDNF-immunoreactive cells in the taste buds were reactive with anti-NCAM or anti-PGP 9.5 antibody, but could be stained with anti-alpha-gustducin antibody. On the other hand, almost all anti-PGP 9.5- or anti-alpha-gustducin-immunoreactive cells were positive for GFR alpha 1. Thus, GDNF-immunoreactive cells did not include...Continue Reading

References

Mar 1, 1975·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·M Takeda, T Hoshino
Jan 1, 1989·Anatomy and Embryology·O B TerkelsenK Møllgård
Nov 1, 1985·Experimental Cell Research·G M Edelman
Jan 1, 1985·The American Journal of Anatomy·A I FarbmanA Nelson
Jan 1, 1973·Histochemie. Histochemistry. Histochimie·H G Geerdink, J Drukker
Nov 1, 1970·The Anatomical Record·S Fujimoto, R G Murray
Jun 1, 1969·Journal of Ultrastructure Research·R G MurrayS Fujimoto
Nov 14, 1983·Brain Research·R J ThompsonJ Rode
Jan 1, 1980·Cell and Tissue Research·M Takeda, K Kitao
Oct 22, 1993·The Journal of Comparative Neurology·G M Nelson, T E Finger
Jun 27, 1996·Nature·G T WongR F Margolskee
Nov 1, 1996·Cell and Tissue Research·K Unsicker
Feb 4, 1999·Annals of the New York Academy of Sciences·B Oakley
Apr 2, 1999·Journal of Electron Microscopy·M TakedaW Breipohl
Mar 12, 2003·The Journal of Comparative Neurology·Cindy L YeeThomas E Finger
Apr 22, 2003·Archives of Histology and Cytology·Nobuhiko UchidaMasako Takeda

❮ Previous
Next ❯

Citations

Jul 25, 2009·Cell and Tissue Research·Akira Ito, Christopher A Nosrat
Oct 12, 2005·Journal of Neurocytology·Irina V NosratChristopher A Nosrat
Jul 15, 2006·Journal of Neurocytology·Gabriella SekerkováJames R Bartles
Sep 30, 2009·In Vitro Cellular & Developmental Biology. Animal·Shin-ichi NakamuraTetsuya Ookura
Jan 9, 2007·BMC Neuroscience·Huazhi MaJohn C Kinnamon
Mar 1, 2006·Archives of Histology and Cytology·Masako TakedaHiroaki Tsunekawa
Jun 11, 2017·Molecular and Cellular Neurosciences·Tao TangRobin Krimm
Apr 3, 2012·The European Journal of Neuroscience·Yoshitaka OhtuboKiyonori Yoshii

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.