PMID: 9177867Jun 1, 1997Paper

Expression of gelatinases within the trabecular bone compartment of ovariectomized and parathyroidectomized adult female rats

Bone
J P MansellA J Bailey

Abstract

Ovariectomized (ovx) and parathyroidectomized (ptx) rat models of disturbed bone metabolism have been widely used in evaluating bone changes resulting from hormonal depletion, and are characterized by elevated and depressed bone turnover, respectively. We report here the expression of gelatinases extracted from native trabecular bone in these models. Nine-month-old female Sprague-Dawley rats were sacrificed after 3 weeks following ovx or 10 days post ptx to determine the influence of these procedures on the levels of proximal tibial bone tissue gelatinases. Identification and quantitation of these enzymes were performed via gelatin gel zymography of native tissue extracts and laser densitometry of developed gels, respectively. In the ptx model, a reduction in tissue levels of pro- and active-MMP-2 and a 45 kDa activated fragment was seen, whereas ovx exhibited significant increases in these enzymes. The MMPs are therefore clearly under the influence of factors known to modulate bone remodeling in vivo. The study of MMP levels directly extracted from bone using these experimental models may assist in developing management regimes for metabolic bone diseases through the use of drugs aimed at controlling turnover.

References

Aug 1, 1992·Matrix : Collagen and Related Research·J A LorenzoM S Hibbs
Aug 1, 1989·Seminars in Arthritis and Rheumatism·D A Watrous, B S Andrews
Apr 5, 1974·Nature·J N Ryan, J F Woessner
Feb 1, 1969·Journal of the American Geriatrics Society·P D Saville
Nov 6, 1984·Biochimica Et Biophysica Acta·J K HeathJ J Reynolds
Nov 15, 1984·European Journal of Biochemistry·K OtsukaH Limeback
Feb 1, 1995·The Journal of Investigative Dermatology·E C BullenE W Howard
Sep 6, 1994·Annals of the New York Academy of Sciences·P ReponenK Tryggvason
Apr 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A L WucherpfennigP Stashenko
Jan 1, 1993·Connective Tissue Research·A M Bollen, D R Eyre
Dec 1, 1993·Journal of Cellular Biochemistry·A Mauviel
Jan 1, 1997·British Journal of Rheumatology·J P MansellA J Bailey

❮ Previous
Next ❯

Citations

Oct 21, 2000·Mechanisms of Ageing and Development·J P MansellJ R Sandy
Feb 3, 2009·Tissue Engineering. Part B, Reviews·Alexander C AlloriStephen M Warren
May 16, 1998·The Journal of Clinical Investigation·J P Mansell, A J Bailey
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M H WassenR A Bank
Jan 26, 2005·Journal of Dental Research·M HolzhausenN Vergnolle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.