Expression of genes coding for antioxidant enzymes and heat shock proteins is altered in primary cultures of rat hepatocytes

Journal of Cellular Physiology
H Van RemmenA Richardson

Abstract

The expression of genes for heat shock proteins in the HSP70 family and genes for antioxidant enzymes was studied in rat hepatocytes cultured in either L-15 or Williams E media on a collagen matrix for up to 48 hours. The mRNA transcripts for the heat shock proteins hsp70, hsc70, and grp78 were induced dramatically when hepatocytes were cultured in L-15, and to a lesser extent when cultured in Williams E. The increase in hsp70 and hsc70 mRNA levels in the cultured hepatocytes was correlated with an increase in the nuclear transcription of these two genes and the binding activity of the heat shock transcription factor to the heat shock element. Culturing rat hepatocytes in either L-15 or Williams E resulted in a decrease in the levels of the mRNA transcripts for catalase and glutathione peroxidase and the activities of these two enzymes. However, the expression of Cu/Zn-superoxide dismutase, i.e., the level of the mRNA transcript or the enzymatic activity, did not change appreciably when hepatocytes were cultured for up to 48 hours. The decline in catalase and glutathione peroxidase expression in the cultured hepatocytes was correlated with a decrease in the GSH/GSSG ratio and an increase in lipid peroxidation. These data show t...Continue Reading

References

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Citations

Jan 22, 1998·Free Radical Biology & Medicine·E Röhrdanz, R Kahl
Mar 4, 2000·Free Radical Biology & Medicine·C C ConradA Richardson
Aug 2, 2003·Biotechnology Progress·Qin Meng
May 31, 2001·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·D M HallK C Kregel
Jun 20, 2006·Toxicology·Maria João Santos-MarquesMaria de Lourdes Bastos
Dec 9, 2010·Human & Experimental Toxicology·Leo Lap Yan WongJennifer Man-Fan Wan
Jul 2, 1998·Journal of Cellular Physiology·Z GuoA Richardson
Mar 2, 1999·Archives of Biochemistry and Biophysics·H Van RemmenA Richardson
Oct 29, 1998·Mechanisms of Ageing and Development·P L MoteS R Spindler
Jun 9, 1999·Mechanisms of Ageing and Development·A Gutsmann-ConradA Richardson

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