Expression of genes containing the IgH enhancer in non-lymphoid cells

Molecular Immunology
B Y Klein, S L Morrison

Abstract

Nuclear run-on experiments were used to verify the hypothesis that extinction of expression of Ig synthesis in L cell x myeloma hybrids occurs at the transcriptional level. Both the H chain enhancer and promoter have been shown to be the targets for extinction in myeloma x T cell hybrids. To examine the expression of genes containing the immunoglobulin heavy chain gene (IgH) enhancer in stably transfected non-B cells, we used a vector with two selectable markers, one of which (gpt providing resistance to mycophenolic acid) either lacks an enhancer or contains the IgH enhancer, the other (neo providing resistance to G418) contains an SV40 enhancer. Stable transfectants of both myeloma (J558L) and L cells selected using G418 were tested to determine if they are also mycophenolic acid resistant. When the IgH enhancer is positioned 3' to the gpt gene, transfected J558L are mycophenolic acid resistant whereas stably transfected L-cells are mycophenolic acid sensitive. However, when large numbers of L cell transfectants are exposed to mycophenolic acid for a prolonged period, resistant subclones emerge. When the 700-bp IgH enhancer fragment was used, the majority of the subclones examined had amplified the vector, between 3 and 38 co...Continue Reading

References

Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A J Berk, P A Sharp
Sep 1, 1987·European Journal of Immunology·D KitamuraT Watanabe
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V T OiP Berg
Nov 1, 1981·Somatic Cell Genetics·M R Clark, C Milstein

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.