Expression of genes controlling steroid metabolism and action in granulosa-lutein cells of women with polycystic ovaries.

Molecular and Cellular Endocrinology
A LernerStephen Franks

Abstract

Aberrant function of granulosa cells has been implicated in the pathophysiology of PCOS. Granulosa lutein (GL) cells were collected during oocyte retrieval for IVF/ICSI. RT-qPCR was used to compare gene expression between 12 control women, 12 with ovulatory PCO and 12 with anovulatory PCOS. To examine which genes are directly regulated by androgens, GL cells from an additional 12 control women were treated in-vitro with 10 nM dihydrotestosterone (DHT). GL cells from women with PCOS showed reduced expression of CYP11A1 3-fold (p = 0.005), HSD17B1 1.8-fold (p = 0.02) and increased expression of SULT1E1 7-fold (p = 0.0003). Similar results were seen in ovulatory women with PCO. GL cells treated with 10 nM DHT showed a 4-fold (p = 0.03) increase in expression of SULT1E1 and a 5-fold reduction in SRD5A1 (p = 0.03). These findings support the notion that aberrant regulation of steroid metabolism or action play a part in ovarian dysfunction in PCOS.

Citations

Jul 6, 2019·The Journal of Clinical Endocrinology and Metabolism·Lisa Ann OwensStephen Franks
Nov 17, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xin ZengZhong-Cheng Mo
Oct 29, 2020·Reproduction in Domestic Animals = Zuchthygiene·Xiaolong YuanJiaqi Li
Mar 31, 2021·Expert Opinion on Therapeutic Patents·Hanan S AnbarMohammed I El-Gamal
Aug 20, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chang-Zhu PeiKwang-Hyun Baek
Jan 28, 2022·Reproductive Sciences·Shikha Rani, Piyush Chandna

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