Expression of genes for collagen production and NF-kappaB gene activation of in vivo healing flexor tendons

The Journal of Hand Surgery
Jin Bo TangXiao Tian Wang

Abstract

Collagen production and reorientation are core events in the healing of tendons and are essential for gaining tensile strength. Intrasynovial flexor tendons are composed chiefly of type I collagen; however, type III collagen is the earliest to be laid down in the wounds. We investigated the expression of genes for type I collagen, type III collagen and its promoter, and nuclear transcription factor kappa B (NF-kappaB) in an in vivo tendon injury model in chickens. Flexor digitorum profundus tendons of the long toes of 12 white leghorn chickens were transected partially and repaired with the modified Kessler method. The repaired tendon segments were harvested at 2, 4, and 8 weeks after surgery with 4 chickens used each time. The uninjured tendons from 4 other chickens were used as the controls. We determined the expression of the collagen and NF-kappaB genes in the tendons with reverse transcription and polymerase chain reaction and semiquantitative analysis of gene bands in gel electrophoresis. Expression of the type III collagen gene was enhanced significantly in the tendons at 2, 4, and 8 weeks after repair. Levels of expression of the type III collagen promoter gene were increased significantly in the healing tendons at 2 an...Continue Reading

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Citations

Nov 16, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Lidiane Isabel FilippinRicardo Machado Xavier
May 11, 2005·Hand Clinics·Jin Bo Tang
Jul 14, 2004·The Journal of Hand Surgery·Charles Hsu, James Chang
Dec 30, 2014·Advanced Drug Delivery Reviews·Diana GasparDimitrios Zeugolis
Jul 19, 2008·Mechanisms of Ageing and Development·William R Swindell
Jul 10, 2012·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Cagri A UysalHiroshi Mizuno
Oct 29, 2013·The Journal of Hand Surgery, European Volume·O A BranfordK J Rolfe
Jun 27, 2006·Lasers in Surgery and Medicine·Carem Fetter RizziJavier González-Gallego
Oct 1, 2005·Lasers in Surgery and Medicine·Lidiane Isabel FillipinJavier González-Gallego
Apr 25, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Hallie E BrinkSteven B Nicoll
Jan 1, 2016·Journal of Biomedical Optics·Marina CurraManoela Domingues Martins
Jun 29, 2021·Journal of Diabetes and Metabolic Disorders·Maryam AminiMohammadreza Amini

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