Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus-oocyte complexes during in vitro maturation and fertilization

Reproduction : the Official Journal of the Society for the Study of Fertility
F NuttinckBenedicte Grimard

Abstract

Prostaglandin E(2) (PGE(2)) and progesterone appear to be critical mediators of cumulus expansion and the resumption of oocyte meiosis. The aim of this study was to identify the types of prostaglandin E synthase (PTGES) expressed in the bovine cumulus-oocyte complex (COC), to characterize their temporal expression during the periconceptional interval using an in vitro model of maturation (IVM) and fertilization (IVF), and to compare their expression with the level of steroidogenic gene expression. Real-time RT-PCR analysis revealed that enzymes related to the PGE(2) biosynthesis pathway were mainly expressed during IVM. Transcripts encoding PTGES1-3 were detected in bovine COCs. Only the expression of PTGES1 significantly increased during IVM whereas that of PTGES2 and PTGES3 remained unchanged. The induction of PTGES1 expression paralleled the induction of prostaglandin G/H synthase-2 (PTGS2) expression and the amounts of PGE(2) secreted by maturing COCs. Concomitantly, cholesterol side chain cleavage cytochrome P450 expression was significantly upregulated in maturing COCs and the high level of expression persisted in fertilized COCs. The expression of the StAR protein remained constant during IVM and then decreased significa...Continue Reading

References

Jan 1, 1992·Archives of Histology and Cytology·S MakabeP M Motta
Jan 1, 1988·Anatomy and Embryology·P HyttelT Greve
Aug 1, 1993·Prostaglandins·W J MurdochL A McPherson
Jun 4, 1999·Reproduction, Fertility, and Development·G FamiliariP M Motta
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·H HizakiA Ichikawa
Jun 30, 2000·Human Reproduction Update·R Canipari
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·J A ElvinM M Matzuk
Dec 6, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·J J Eppig
Jan 5, 2002·Molecular Reproduction and Development·F NuttinckG Charpigny
Feb 9, 2002·Molecular Reproduction and Development·Sofie TangheAart de Kruif
Apr 25, 2003·The Journal of Steroid Biochemistry and Molecular Biology·M SchoenfelderR Einspanier
Nov 26, 2003·Progress in Lipid Research·Makoto Murakami, Ichiro Kudo
Apr 20, 2005·Molecular and Cellular Endocrinology·A TsafririS H Pomerantz
Dec 13, 2005·Journal of Biochemistry and Molecular Biology·Ichiro Kudo, Makoto Murakami
Feb 7, 2006·Reproductive Biology and Endocrinology : RB&E·Hai Feng WangTakato Terada
Mar 17, 2006·Molecular Reproduction and Development·Makoto OrisakaFumikazu Kotsuji
Oct 31, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Cecilia BallaréMiguel Beato

❮ Previous
Next ❯

Citations

Jan 15, 2011·Zygote : the Biology of Gametes and Early Embryos·Marie Saint-DizierAndrew A Ponter
Oct 5, 2010·Reproduction, Fertility, and Development·Mohamad SalhabSvetlana Uzbekova
Mar 11, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Waleed F MareiAli A Fouladi-Nashta
Sep 13, 2014·Theriogenology·Irina Y LebedevaMette Schmidt
Jan 23, 2014·Reproductive Biomedicine Online·Waleed F MareiAli A Fouladi-Nashta
Jul 10, 2009·Biology of Reproduction·Waleed F MareiAli A Fouladi-Nashta
Jan 19, 2018·International Journal of Molecular Sciences·Eva Nagyova
May 13, 2020·Reproductive Biology and Endocrinology : RB&E·Dorota BoruszewskaIzabela Woclawek-Potocka
Jan 27, 2021·Journal of Experimental & Clinical Cancer Research : CR·Sinae KimSuhwan Chang
Dec 2, 2019·Animal Reproduction Science·Diego Fernando Dubeibe MarinOtavio Mitio Ohashi
Feb 11, 2021·Reproduction in Domestic Animals = Zuchthygiene·Juliana de Carvalho DelgadoMayra Elena Ortiz D'Ávila Assumpção
Apr 24, 2021·Zygote : the Biology of Gametes and Early Embryos·Eryk AndreasDawit Tesfaye
Dec 30, 2021·Animal Biotechnology·Nayara Ribeiro KussanoMargot Alves Nunes Dode

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.