Expression of germ cell nuclear factor (GCNF/RTR) during spermatogenesis

Molecular Reproduction and Development
Y L ZhangD A O'Brien

Abstract

Germ cell nuclear factor (GCNF/RTR), a novel orphan receptor in the nuclear receptor superfamily of ligand-activated transcription factors, is expressed predominantly in developing germ cells. In several mammalian species two GCNF/RTR mRNAs are present in the testis, with the smaller 2.3-kb transcript generally expressed at higher levels than the larger 7.4- or 8.0-kb transcript. In both the mouse and rat, the 2.3- and 7.4-kb GCNF/RTR transcripts were detected in isolated spermatogenic cells, but not in Sertoli cells. Expression of these transcripts is differentially regulated, with the larger 7.4-kb mRNA appearing earlier during testicular development. The major 2.3-kb transcript is expressed predominantly in round spermatids in the mouse and rat. In situ hybridization studies in the rat demonstrated that GCNF/RTR transcripts reach maximal steady-state levels in round spermatids at stages VII and VIII of the spermatogenic cycle, and then decline abruptly as spermatids begin to elongate. RNase protection assays were used to predict the 3' termination site of the 2.3-kb transcript. An alternative polyadenylation signal (AGUAAA) was identified just upstream of this termination site. These studies suggest that GCNF/RTR may regulat...Continue Reading

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Citations

Feb 28, 2003·Biology of Reproduction·Genyan YangDeborah A O'Brien
Sep 5, 2003·Biology of Reproduction·Yong-Sik KimAnton M Jetten
Jan 24, 2009·Biology of Reproduction·Zi-Jian LanAustin J Cooney
Jul 19, 2000·Genome Research·E BeaudoingD Gautheret
Feb 24, 2001·Genome Biology·U Süsens, U Borgmeyer
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A M WallaceC C MacDonald
Dec 18, 2007·Biochemical and Biophysical Research Communications·Heng ZhangZi-Jian Lan
Dec 19, 2009·Molecular Reproduction and Development·Sang In LeeJae Yong Han
Dec 4, 2003·Molecular Reproduction and Development·Alexandra Zilz, Geoffrey M Cooper
Dec 11, 2003·Genesis : the Journal of Genetics and Development·Zi-Jian LanAustin J Cooney
Jul 6, 2004·Molecular Reproduction and Development·Geoffrey C Hummelke, Austin J Cooney
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Jun 24, 2017·Cellular and Molecular Life Sciences : CMLS·Dimitrios GkikasPanagiotis K Politis
Oct 17, 2002·The Journal of Biological Chemistry·Zi-Jian LanAustin J Cooney
Mar 1, 2020·Animals : an Open Access Journal From MDPI·Zhicheng ShiFangxiong Shi
Jun 25, 2002·The Journal of Biological Chemistry·Zhijiang YanAnton M Jetten
Aug 21, 2014·PloS One·Davood SabourAustin J Cooney

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