Expression of glial cell-derived neurotrophic factor and its receptor in the stem-cell-containing human limbal epithelium.

The British Journal of Ophthalmology
Hong QiStephen C Pflugfelder

Abstract

To evaluate the expression pattern of glial cell line-derived neurotrophic factor (GDNF) with its receptors GDNF family receptor alpha-1 (GFR alpha-1) and Ret in the human corneal and limbal tissues, as well as in the primary human limbal epithelial cultures (PHLEC). Expression of GDNF and its receptors, and the co-localisation with stem cell associated and differentiation markers were evaluated by immunofluorescent staining, western blot analysis and real-time PCR in the fresh human corneoscleral tissues, as well as in the PHLEC. Single cell colony-forming and wound-healing assays were also evaluated in PHLEC. GDNF and GFR alpha-1 were found to be expressed by a subset of basal cells and co-localised with ATP-binding cassette, subfamily G (WHITE), member 2 (ABCG2) and p63, but not with cytokeratin 3 in the human limbal basal epithelium. In PHLEC, they were expressed by a small population of cells in the less differentiated stage. The GDNF and GFR alpha-1-positive subpopulations were enriched for the expression of ABCG2 and p63 (p<0.01). Recombinant human GDNF promoted the proliferation and wound healing of epithelial cells in the PHLEC. In contrast, Ret was abundantly located in the human corneal epithelium except for the basa...Continue Reading

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Citations

Apr 30, 2014·Journal of Cellular Physiology·Marta RusminiIsabella Ceccherini
Jan 8, 2016·The Ocular Surface·Robert IhnatkoNeil Lagali
Apr 29, 2015·Progress in Retinal and Eye Research·Nick Di Girolamo
Jan 18, 2016·The Ocular Surface·Alexander RichardsonNick Di Girolamo
Apr 7, 2010·The International Journal of Biochemistry & Cell Biology·Fang BianDe-Quan Li
Mar 25, 2010·Biochemical and Biophysical Research Communications·Naoyuki MorishigeTeruo Nishida
Nov 28, 2018·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·S MertschG Geerling
Aug 29, 2020·International Journal of Molecular Sciences·Raquel Hernáez-MoyaNoelia Andollo

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