Expression of Glutamate Receptor Subtype 3 Is Epigenetically Regulated in Podocytes under Diabetic Conditions

Kidney Diseases
Zhengzhe LiJohn Cijiang He

Abstract

Recent studies suggest a role of epigenetics in the pathogenesis of diabetic kidney disease. However, epigenetic changes occurring specifically in kidney cells is poorly understood. To examine the epigenetic regulation of genes in podocytes under diabetic conditions, we performed DNA methylation and transcriptomic profiling in podocytes exposed to high glucose conditions. Comparative analysis of genes with DNA methylation changes and correspondingly altered mRNA expression identified 337 hypomethylated genes with increased mRNA expression and only 2 hypermethyated genes (ESX1 and GRIA3) with decreased mRNA expression. Glutamate ionotropic receptor AMPA type subunit 3 (GRIA3) belongs to the ionotropic class of glutamate receptors that mediate fast excitatory synaptic transmission in the central nervous system. As podocytes have glutamate-containing vesicles and various glutamate receptors mediate important biological effects in podocytes, we further examined GRIA3 expression and its function in podocytes. Real-time PCR and western blots confirmed the suppression of GRIA3 expression in podocytes under high glucose conditions, which were abolished in the presence of a DNA methyltransferase inhibitor. Sites of DNA hypermethylation ...Continue Reading

References

Jan 15, 1997·The Journal of Clinical Investigation·M E PagtalunanT W Meyer
May 31, 2001·Kidney International·M W SteffesUNKNOWN International Diabetic Nephropathy Study Group
Dec 20, 2003·Annals of the New York Academy of Sciences·Robert H Lipsky, David Goldman
Jan 4, 2005·Nephron. Experimental Nephrology·Arndt T PetermannStuart J Shankland
Feb 5, 2005·Developmental Cell·M Albert BassonJonathan D Licht
Jan 10, 2006·The American Journal of Pathology·Daniela MacconiAndrea Remuzzi
Jan 13, 2006·Journal of the American Society of Nephrology : JASN·Katalin Susztak, Erwin P Böttinger
Apr 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria Pia RastaldiGiuseppe D'Amico
Nov 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ye WuTao Wang
Mar 26, 2008·Biochemical and Biophysical Research Communications·Junta NakajimaMasanori Hatakeyama
Jul 7, 2009·Journal of the American Society of Nephrology : JASN·Laura GiardinoMaria P Rastaldi
Aug 7, 2010·Neoplasia : an International Journal for Oncology Research·Stefanie RipkaPatrick Michl
Mar 2, 2011·The American Journal of Pathology·Aldamaria PulitiMaria Pia Rastaldi
Sep 11, 2012·Seminars in Nephrology·Pierre-Louis Tharaux, Tobias B Huber
Sep 15, 2012·Annual Review of Physiology·Janesh Kumar, Mark L Mayer
Nov 21, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Laura J SmythAmy Jayne McKnight
Jul 9, 2014·Nature Reviews. Nephrology·Mitsuo Kato, Rama Natarajan
Apr 2, 2015·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Stuart E Dryer
Apr 24, 2015·Molecular Pharmacology·Hongjie YuanStephen F Traynelis
Aug 13, 2015·Journal of the American Society of Nephrology : JASN·Jia FuJohn Cijiang He
May 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zhuo ChenRama Natarajan

❮ Previous
Next ❯

Citations

Jun 13, 2020·International Journal of Molecular Sciences·Julio M Martinez-MorenoAna B Sanz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis