PMID: 8950186Nov 11, 1996Paper

Expression of glutamyl-tRNA reductase in Escherichia coli

Biochimica Et Biophysica Acta
W ChenS Lee

Abstract

The biosynthesis of the hemes, chlorophylls, corrins and other tetrapyrroles begins with the synthesis of 5-aminolevulinic acid (ALA). The pathway is highly conserved except for the synthesis of ALA which is derived from glycine and succinyl CoA (C4) in most eukaryotes and from glutamate (C5) in most bacteria and in green plants. In C5, glutamyl-tRNA synthetase (GTS) converts glutamate to glutamyl-tRNA (glu-tRNA), which is reduced by glutamyl-tRNA reductase (GTR) to glutamyl-1-semialdehyde (GSA), which is converted by aminotransferase (GSA-AT) to ALA. Since GTS is also involved in protein synthesis and GSA can be converted to ALA non-enzymatically, it is highly probable that control of ALA synthesis and thus of the whole pathway resides in the GTR step. In Escherichia coli, GTR is the gene product of hemA. BL21(DE3), a protease-deficient strain which contains the T7 RNA polymerase gene in front of a lac promoter, was transformed with a pET14b-based vector, pWC01, harboring hemA in front of a T7 promoter and ORF1 which is transcribed in the opposite direction. The transformed strain, WC1201, secreted ALA and porphyrins into the medium. Induction of expression of hemA by WC1201 was optimized for concentration of inducer (IPTG, 5 ...Continue Reading

References

Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S I BealeS Granick
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Oct 1, 1989·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·S LabergeJ Lapointe
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C T ChungR H Miller
May 1, 1989·Journal of Bacteriology·J M LiC S Russell
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M SpringerM Grunberg-Manago
Jan 1, 1985·Methods in Enzymology·P Hoben, D Söll
Apr 1, 1986·Archives of Biochemistry and Biophysics·T Oh-hamaS Miyachi
Apr 24, 1968·Biochimica Et Biophysica Acta·S S Bottomley, G A Smithee
Apr 1, 1972·Archives of Biochemistry and Biophysics·R J PorraA M Tennick
May 1, 1994·Journal of Bacteriology·W ChenS D Cosloy
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·R R RadtkeyT J Case
May 1, 1999·The Journal of Biological Chemistry·K Y LeeG D Rosen
Dec 1, 1955·The Biochemical Journal·K D GIBSONJ J SCOTT
May 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Daniel P KennedyEric Courchesne
Jun 23, 2006·Infection and Immunity·Marcia M JumblattDonald R Demuth

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Citations

Oct 11, 2003·Protein Expression and Purification·Stefan SchauerJürgen Moser
Jan 27, 2017·Microbiology and Molecular Biology Reviews : MMBR·Harry A DaileyMartin J Warren
Oct 9, 2002·The Journal of Biological Chemistry·Stefan SchauerDieter Jahn

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