Expression of glycine receptor subunits and gephyrin in single bipolar cells of the rat retina

Visual Neuroscience
R Enz, J Bormann

Abstract

We studied the expression of glycine receptor (GlyR) subunits and gephyrin in the adult rat retina. Reverse transcribed RNA was amplified by polymerase chain reaction (RT-PCR) with primers designed to recognize GlyR alpha 1, alpha 2, alpha 3, beta subunits, and gephyrin. Using RNA isolated from the whole retina, signals for all four GlyR subunits and gephyrin could be observed. In rod bipolar cells, in contrast, we detected a subset of GlyR subunits, alpha 1 and beta, and no gephyrin. Patch-clamp recording employing two subtype-specific blockers of the GlyR, picrotoxinin and cyanotriphenylborate (CTB), indicated that the GlyR in rod bipolar cells is a heteromeric protein composed of the alpha 1 and beta subunit. Moreover, the absence of detectable amounts of gephyrin mRNA suggests that the anchor protein is not required for the function of GlyRs in rod bipolar cells.

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Citations

Oct 26, 1999·Progress in Retinal and Eye Research·M Kalloniatis, G Tomisich
Mar 9, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Regina D NoblesMaureen A McCall
Dec 3, 2003·The Journal of Physiology·Yi HanMalcolm M Slaughter
Jul 1, 1995·The European Journal of Neuroscience·R EnzJ Bormann
Jul 1, 2009·The Journal of Physiology·Lei DuanMalcolm M Slaughter
Sep 17, 2003·The Journal of Comparative Neurology·Silke HaverkampHeinz Wässle
Jan 20, 2006·The European Journal of Neuroscience·Elena IvanovaHeinz Wässle
Jul 17, 1998·Vision Research·R Enz, G R Cutting
Jul 17, 1998·Vision Research·S M Wu, B R Maple
Jul 17, 1998·Vision Research·H WässleC M Becker
Aug 3, 2002·The European Journal of Neuroscience·G GisselmannJ Bormann
Mar 17, 2010·Biochemical and Biophysical Research Communications·Kyunghee X Kim, Daniel C Marcus
Dec 14, 1999·The Journal of Comparative Neurology·S YazullaD G Deutsch
Sep 24, 2004·Physiological Reviews·Joseph W Lynch

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