PMID: 2502171May 2, 1989Paper

Expression of Go alpha mRNA and protein in bovine tissues

Biochemistry
S R PriceM Vaughan

Abstract

Go alpha is a 39-kDa guanine nucleotide-binding protein (G protein) similar in structure and function to Gs alpha and Gi alpha of the adenylate cyclase complex and to transducin (Gt alpha) of the retinal photon receptor system. Although expression of Go alpha protein has been reported to be tissue-specific, other workers have found Go alpha mRNA in all rat tissues examined. In order to clarify this contradiction, studies to verify the distribution of Go alpha mRNA and protein in bovine and rat tissues were performed. Tissues were screened for the presence of Go alpha mRNA by use of a series of restriction fragments of a bovine retinal cDNA clone, lambda GO9, and oligonucleotide probes complementary to sequences specific among G alpha subunits for the 5' untranslated and coding regions of Go alpha. These probes hybridized predominantly with mRNA of 4.0 and 3.0 kb in bovine brain and retina. A 2.0-kb mRNA in retina also hybridized strongly with the cDNA but weakly with the oligonucleotide probes. In bovine lung, two mRNAs of 1.6 and 1.8 kb hybridized with the cDNA while only the 1.6-kb species hybridized with the coding-region oligonucleotide. In bovine heart, only a 4.0-kb mRNA was detected and in amounts much less than those in...Continue Reading

References

Jan 1, 1986·Annual Review of Neuroscience·L Stryer
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Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
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Citations

Jan 1, 1994·Life Sciences·C W EmalaM A Levine
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T TsukamotoY Kaziro
Feb 28, 1992·Biochemical and Biophysical Research Communications·T B HaugenV Hansson
Dec 7, 2007·Biochemical and Biophysical Research Communications·Atsushi Fujita
Dec 19, 2019·Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·S B Izvozchikov
Apr 11, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lei LuW Allan Walker

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