Expression of GOLPH3 in patients with non-small cell lung cancer and xenografts models

Oncology Letters
Shicong TangJianlun Liu

Abstract

Increased expression of Golgi phosphoprotein 3 (GOLPH3) has been reported to be associated with several types of human cancer. Patient-derived cancer xenograft models have demonstrated great potential in preclinical studies. In the present study, the link between GOLPH3 expression and survival was examined in patients with non-small cell lung cancer (NSCLC). Patient-derived lung cancer xenograft models were established with two different methods. Lastly, the association between GOLPH3 expression and establishment of the xenograft models was explored. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry analysis were used to examine GOLPH3 expression in 60 NSCLC tissues and matched adjacent non-cancerous tissues (ANT). In addition, tumor pieces from the 60 NSCLC tissues were implanted in the subcutaneous layer and in the subrenal kidney capsule of nude mice. RT-qPCR, histopathology and immunohistochemistry were used to confirm the human origin of the xenograft tumors. RT-qPCR was also used to research the mutation status of GOLPH3 in the xenograft tumors. The results demonstrated that NSCLC tissues had higher expression of GOLPH3, at the mRNA and protein level, compared with ANT. High e...Continue Reading

Citations

Jan 11, 2020·Frontiers in Oncology·Li-Na YuXing Hua
May 27, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Zhong-Shi HongJian-Peng Pan

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Methods Mentioned

BETA
xenograft
xenografts
biopsy

Software Mentioned

AVMA
SPSS
ARRIVE

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