Expression of gonococcal protein II in Escherichia coli by translational fusion

Molecular Microbiology
L PalmerS Falkow

Abstract

A protein II (P.II) gene from Neisseria gonorrhoeae was cloned in Escherichia coli and characterized by DNA sequence analysis. As with other reported P.II sequences, this gene contains an ATG initiation codon which is out of frame with respect to the remainder of the P.II amino acid sequence. A translational fusion was constructed in E. coli which linked the P.II sequence to the signal peptide of beta-lactamase. This P.II fusion differs from the gonococcal protein only in the first seven residues at the N terminus. In E. coli, the P.II fusion product exhibits properties analogous to those of P.II in N. gonorrhoeae. The P.II fusion product is a major component of the E. coli outer membrane and it is exposed on the cell surface. The P.II fusion protein also exhibits the heat-modifiable phenotype of gonococcal P.II.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R P Ambler, G K Scott
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J G Sutcliffe
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D Pribnow
Sep 1, 1985·Infection and Immunity· Newhall WJ5thR B Jones
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Manoil, J Beckwith
Jul 1, 1986·Journal of Bacteriology·R S Schwalbe, J G Cannon
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N H Carbonetti, P F Sparling
Nov 22, 1965·Biochemical and Biophysical Research Communications·L Leive
Aug 1, 1984·Journal of Bacteriology·P E Orndorff, S Falkow
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K TalmadgeW Gilbert
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Feb 1, 1981·Journal of Bacteriology·J E Heckels
Jul 1, 1982·Infection and Immunity·J Swanson
Jul 1, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·J E ButteryP R Pannall

❮ Previous
Next ❯

Citations

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D Simon, R F Rest
Jun 1, 1991·Molecular Microbiology·E L AhoJ G Cannon
Jul 1, 1992·Molecular Microbiology·R J BellandS H Fischer
Sep 1, 1991·Infection and Immunity·P van der LeyJ T Poolman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.