Expression of granzyme A and perforin in mouse heart transplants immunosuppressed with donor-specific transfusion and anti-CD4 monoclonal antibody

Transplantation
R H ChenP J Morris

Abstract

Granzyme A and perforin are produced by activated cytotoxic T lymphocytes and their expression correlates with the appearance of cytotoxicity. Using in situ hybridization and immunohistochemistry, we examined the phenotype of cellular infiltration and the appearance of granzyme A+ and perforin+ cells in a mouse cardiac transplant model where the recipients were pretreated with donor-specific transfusion, anti-CD4 monoclonal antibody, or both. While the profiles of cellular infiltration failed to correlate with graft survival, tolerized grafts, as compared with untreated allografts, showed a decreased frequency of granzyme A and perforin expression. These functional markers of cytotoxic T lymphocytes can differentiate between rejecting and indefinitely surviving grafts and may be of value in dissecting the immunological events involved in tolerance induction.

References

Jun 12, 1975·The New England Journal of Medicine·T B StromG J Busch
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·L E Fields, D Y Loh
Dec 1, 1990·European Journal of Immunology·S X QinH Waldmann
Mar 1, 1991·European Journal of Immunology·G M GriffithsI Weissman
Sep 1, 1988·Transplantation·W N PeughP J Morris

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Citations

Jul 22, 1998·Journal of Neuroimmunology·K KogureY Matsumoto
Jun 25, 2004·The American Journal of Pathology·Jonathan C ChoyDavid J Granville
Jan 1, 1996·Advances in Nephrology From the Necker Hospital·P DruetL Pelletier
Apr 15, 1997·Transplantation·K R AhmedK K Gaal

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