Expression of Gre2p improves tolerance of engineered xylose-fermenting Saccharomyces cerevisiae to glycolaldehyde under xylose metabolism

Applied Microbiology and Biotechnology
Lahiru N JayakodyYong-Su Jin

Abstract

Engineered S. cerevisiae employing the xylose reductase pathway enables efficient xylose valorization to fuels and chemicals. However, toxicity of thermochemically pretreated biomass hydrolysate on S. cerevisiae is one of the key technical challenges to upgrade biomass-derived sugars including xylose and glucose into high-value products. We investigated the effect of glycolaldehyde, one of the biomass-derived highly toxic aldehyde compounds, and its combinatorial inhibitory effect with other major fermentation inhibitors commonly found in plant hydrolysate such as methylglyoxal, 5-HMF, furfural, vanillin, and acetic acid on engineered xylose-fermenting S. cerevisiae in xylose and/or glucose media. We elucidated that glycolaldehyde and methylglyoxal are the key inhibitory short-aliphatic aldehydes on engineered xylose-fermenting S. cerevisiae in xylose-containing medium. Indeed, the degree of toxicity of these tested fermentation inhibitors varies with the sole carbon source of the medium. We demonstrate that genome integration of an extra copy of autologous GRE2 with its native promotor substantially improved the toxic tolerance of engineered xylose-fermenting S. cerevisiae to major inhibitory compounds including glycolaldehyde...Continue Reading

References

May 1, 1993·Biochemical Society Transactions·A Kimura, Y Inoue
Jul 17, 1999·Yeast·A Garay-Arroyo, A A Covarrubias
Jun 21, 2002·Methods in Enzymology·R Daniel Gietz, Robin A Woods
Apr 7, 2006·Yeast·Jonas Warringer, Anders Blomberg
Jun 6, 2008·Microbial Cell Factories·Laura SalusjärviLaura Ruohonen
Nov 8, 2011·Chemical Research in Toxicology·Richard M LopachinDavid S Barber
Feb 9, 2012·Applied Microbiology and Biotechnology·Lahiru N JayakodyHiroshi Kitagaki
Mar 13, 2013·The Journal of Physical Chemistry. a·Jeremy KuaDavid O De Haan
Jun 19, 2013·Molecular Systems Biology·Jeffrey M SkerkerAdam P Arkin
Jun 10, 2014·Chemical Research in Toxicology·Richard M LoPachin, Terrence Gavin
Mar 10, 2016·Critical Reviews in Biotechnology·Lahiru N JayakodyHiroshi Kitagaki
Oct 28, 2016·Biotechnology and Bioengineering·Guo-Chang ZhangYong-Su Jin
Jun 21, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Yun-Cheng LiKenji Kida

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Citations

Nov 22, 2018·Frontiers in Microbiology·Akaraphol WatcharawipasHiroshi Takagi
Nov 7, 2018·Applied Microbiology and Biotechnology·Joana T CunhaLucília Domingues
Mar 21, 2021·Applied Microbiology and Biotechnology·Lahiru N Jayakody, Yong-Su Jin

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Methods Mentioned

BETA
PCR
electrophoresis
protein assay
targeted modification
sumoylation

Software Mentioned

Multibase
MINITAB

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