Expression of growth factor mRNAs by human Tenon's capsule fibroblasts

Experimental Eye Research
R C TripathiB J Tripathi

Abstract

By using the techniques of reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern hybridization, we demonstrated that human Tenon's capsule fibroblasts in primary cultures, express the messenger RNA (mRNA) transcripts encoding transforming growth factor-beta 1 (TGF-beta 1), basic fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF), but not those coding for aFGF and TGF-beta 2. Two PCR products were obtained for TGF-beta 1: a major fragment of 161 base pairs that corresponded to the expected size, and a minor sequence of 400 base pairs. An antisense oligonucleotide probe specific for TGF-beta 1 detected only the band of 161 base pair of PCR-amplified sequence. For bFGF, PDGF-A and PDGF-B, we obtained only a single PCR product with the anticipated length of 222, 225, and 217 base pairs, respectively. Southern blotting experiments revealed that these PCR-amplified fragments were specific for the respective growth factors. The negative control experiments without template did not reveal any amplification. No product for aFGF or TGF-beta 2 was detected. By radioimmunoassay, TGF-beta 1 protein was detected at the level of 24-30 pg ml-1 per 2 million Tenon's fibroblasts during a 24-hour period in ...Continue Reading

Citations

Nov 10, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Lars ChoritzHagen Thieme
Nov 18, 2011·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Xiaoning LiJian Ge
May 6, 2017·International Journal of Molecular Sciences·Michiko MaedaTsunehiko Ikeda
Nov 14, 1997·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·M KnorrH J Thiel
Jun 18, 2020·Clinical Ophthalmology·Charles B TrelfordCindy M L Hutnik
Jul 18, 2003·Current Eye Research·Peter Oliver DenkMarcus Knorr
Sep 25, 2020·Journal of Glaucoma·Jeffrey Freedman
Dec 18, 2020·The British Journal of Ophthalmology·Naveed NilforushanMahammad Banifatemi

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