Expression of growth hormone and its receptor in the lungs of embryonic chicks

Cell and Tissue Research
Jason A BeyeaSteve Harvey

Abstract

The lung is well established as being a postnatal target site for growth hormone (GH) action, since pathophysiological states of GH excess and deficiency are both associated with impaired pulmonary function. Pituitary GH is therefore probably involved in normal lung growth or development, although perinatal lung development occurs prior to the differentiation of pituitary somatotrophs and the ontogeny of pituitary GH secretion. The lung itself may, however, be a site of GH production during prenatal development, since a specific GH-response gene (a marker of GH activity) is expressed in the lungs of early chick embryos, in which GH immunoreactivity is widespread in many other peripheral tissues. We have assessed this possibility in embryonic chicks. A 690-bp cDNA, identical in size and nucleotide sequence to the full-length pituitary GH transcript, was amplified by reverse transcription/polymerase chain reaction from total RNA extracted from the lungs of embryos at 11, 13, 15, and 18 days of the 21-day incubation period. This transcript was localized by in situ hybridization to mesenchymal and epithelial cells of the developing lungs, in which specific GH immunoreactivity was similarly located. Intense GH immunoreactivity was a...Continue Reading

References

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Apr 5, 2002·Journal of Molecular Neuroscience : MN·Steve HarveyMark Pines
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Oct 14, 2004·Anatomy and Embryology·Esmond J Sanders, Steve Harvey

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Citations

Oct 26, 2010·Endocrine·S Harvey
Feb 3, 2011·The Journal of Physiological Sciences : JPS·Mei-Hua JinSusumu Minamisawa
Feb 5, 2013·General and Comparative Endocrinology·Steve Harvey
Nov 6, 2008·Molecular and Cellular Biochemistry·J A BeyeaS Harvey

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