Expression of growth hormone by lymphocytes

International Reviews of Immunology
D A Weigent, J E Blalock

Abstract

In the present study, we evaluated whether mononuclear leukocytes could synthesize and secrete growth hormone (GH) in vitro. Studies using antibody affinity chromatography, high pressure liquid chromatography, and polyacrylamide gel electrophoresis indicate that leukocytes secrete a approximately 22,000 dalton molecular weight immunoreactive GH (irGH). The irGH appeared to be de novo synthesized since it could be radiolabeled with tritiated amino acids and its production blocked by prior incubation of leukocytes with cycloheximide. The levels of secreted irGH were enhanced by concanavalin A or lipopolysaccharide. By using RNA slot blot analysis, we detected specific GH mRNA present in the cytoplasm of rat leukocytes. Leukocytes from a variety of tissues in rats, including spleen, thymus, bone marrow, and peripheral blood as well as separated spleen T and B cells, were all observed to produce GH RNA and secrete GH from the cells. We conclude that lymphocytes produce a mRNA for irGH and are then able to translate that message and secrete the molecule from the cell. The data suggest a potential regulatory loop between the immune and neuroendocrine systems.

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Citations

Oct 1, 1995·Endocrine·C L RenderS Harvey
Oct 26, 2010·Endocrine·S Harvey
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·O LahunaA Mode
Mar 20, 2014·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Diana Cristina Pérez-IbaveHugo Alberto Barrera-Saldaña
Nov 25, 2003·Microscopy Research and Technique·Lourdes Domínguez-Gerpe, Manuel Rey-Méndez
Jan 16, 2009·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Naoki Hattori
Apr 1, 1993·Trends in Endocrinology and Metabolism : TEM·M C Gelato
Nov 15, 2005·Endocrine Journal·Renee J FreedmanMichael T Collins
Jan 12, 2001·Molecular Cell Biology Research Communications : MCBRC·J A CostoyaJ Devesa
Jun 6, 2020·Cells·Sher Bahadur PoudelShoshana Yakar

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