Expression of growth hormone receptors in murine lymphoid cells analyzed by flow cytofluorometry

Endocrinology
M C GagneraultM Dardenne

Abstract

We have analyzed the expression of GH receptors (GHR) in murine lymphoid organs using flow cytofluorometry with biotinylated bovine GH and specific fluorescein isothiocyanate-labeled monoclonal antibodies defining distinct lymphoid cell populations. GHR were widely expressed in al murine hematopoietic tissues and in fetuses, newborns, and 3- and 7-week-old animals. In the bone marrow, all hematopoietic lineages expressed variable levels of GH receptors, whereas in the thymus, this expression was mainly seen in CD4-, CD8-, CD4+CD8+, and CD8+ subpopulations. At the periphery, 50% of splenocytes and peripheral blood lymphocytes and 20% of lymph node cells were GHR positive, with a wider receptor expression on B cells and macrophages (approximately 50%) than on T cells (approximately 20%), where the labeling was seen on both CD4+ and CD8+ cell subsets. Interestingly, the proportion of GHR-bearing CD4+ and CD8+ splenocytes significantly increased after T cell activation with Concanavalin A or anti-CD3. Finally, we demonstrated that all peripheral T cells expressing GHR also expressed PRL receptors. Our study provides a molecular basis to study the factors controlling GHR expression and to better understand the influence of GH in the...Continue Reading

Citations

Mar 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Javier Cote-SierraWilliam E Paul
Nov 5, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Kenneth DorshkindWilliam J Murphy
Apr 27, 2002·Scandinavian Journal of Immunology·W SavinoM Dardenne
Jan 15, 2004·Microscopy Research and Technique·Jonas HannestadJosé A Vega
Jul 18, 2009·Current Opinion in Immunology·Amanda M Holland, Marcel R M van den Brink
Jan 16, 2009·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Naoki Hattori
Jul 18, 2008·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·R NovosyadlyyJ-G Scharf
Jun 10, 2003·Annals of the New York Academy of Sciences·Wilson SavinoMireille Dardenne
Apr 9, 2011·Journal of Neuroimmunology·Judith M Greer, Pamela A McCombe
Feb 22, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S RecherG Morel
Jul 4, 2001·Lupus·Y S BaeS Y Kim
Jan 10, 2017·International Journal of Molecular Sciences·Miłosz Piotr KawaBogusław Machaliński
Oct 7, 2015·Nature Reviews. Endocrinology·Wilson SavinoMireille Dardenne
Aug 19, 2000·Endocrine Reviews·W Savino, M Dardenne
Aug 23, 2000·Leukemia & Lymphoma·L A WelniakW J Murphy
Oct 23, 2002·Journal of Applied Physiology·Gwendolyn F ElphickMonika Fleshner
Aug 12, 2004·The Journal of Biological Chemistry·Ruth GarzónMario Mellado

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