Expression of Haemophilus ducreyi collagen binding outer membrane protein NcaA is required for virulence in swine and human challenge models of chancroid.

Infection and Immunity
Robert A FulcherThomas H Kawula

Abstract

Haemophilus ducreyi, the etiologic agent of the sexually transmitted genital ulcer disease chancroid, has been shown to associate with dermal collagen fibers within infected skin lesions. Here we describe NcaA, a previously uncharacterized outer membrane protein that is important for H. ducreyi collagen binding and host colonization. An H. ducreyi strain lacking the ncaA gene was impaired in adherence to type I collagen but not fibronectin (plasma or cellular form) or heparin. The mutation had no effect on serum resistance or binding to HaCaT keratinocytes or human foreskin fibroblasts in vitro. Escherichia coli expressing H. ducreyi NcaA bound to type I collagen, demonstrating that NcaA is sufficient to confer collagen attachment. The importance of NcaA in H. ducreyi pathogenesis was assessed using both swine and human experimental models of chancroid. In the swine model, 20% of lesions from sites inoculated with the ncaA mutant were culture positive for H. ducreyi 7 days after inoculation, compared to 73% of wild-type-inoculated sites. The average number of CFU recovered from mutant-inoculated lesions was also significantly reduced compared to that recovered from wild-type-inoculated sites at both 2 and 7 days after inoculati...Continue Reading

References

Jul 1, 1992·Microbial Pathogenesis·D AbeckH Mensing
Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Jul 1, 1995·Clinical Microbiology Reviews·D L Trees, S A Morse
Aug 1, 1995·Infection and Immunity·M M HobbsT H Kawula
May 1, 1994·The Journal of Infectious Diseases·S M SpinolaA A Campagnari
Mar 1, 1993·International Journal of Dermatology·H J Engelkens, E Stolz
Feb 1, 1996·The Journal of Infectious Diseases·S M SpinolaA F Hood
Jul 1, 1996·The Journal of Infectious Diseases·S A HienzJ I Flock
Aug 1, 1996·The Journal of Infectious Diseases·R KingJ A Wilkins
May 1, 1996·International Journal of STD & AIDS·J O Ndinya-AcholaK K Holmes
Dec 15, 1996·FEMS Microbiology Letters·C E ThomasP F Sparling
Apr 10, 1997·The New England Journal of Medicine·R A RoyceM S Cohen
Nov 14, 1997·Infection and Immunity·C AebiE J Hansen
Dec 24, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·K E NelsonJ M Zenilman
Nov 17, 1998·The Journal of Infectious Diseases·J A Al-TawfiqS M Spinola
May 4, 1999·Infection and Immunity·M E Bauer, S M Spinola
Aug 17, 1999·Sexually Transmitted Infections·A KamaliJ A Whitworth
Aug 24, 1999·Infection and Immunity·L R San MateoT H Kawula
Sep 9, 1999·The Journal of Infectious Diseases·F M BehetsM S Cohen
Feb 26, 2000·Infection and Immunity·C ElkinsB Olsen
May 19, 2000·Infection and Immunity·M N RhemK R Wilhelmus
Oct 26, 2001·Sexually Transmitted Diseases·E L KorenrompJ D Habbema
May 16, 2002·Infection and Immunity·Joseph R NikaEric J Hansen
Oct 16, 2002·Infection and Immunity·Leah E ColeChristopher Elkins
Feb 11, 2003·Sexually Transmitted Infections·D A Lewis

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Citations

Apr 22, 2008·The Journal of Infectious Diseases·Keith E BanksStanley M Spinola
May 13, 2009·The Journal of Infectious Diseases·Diane M JanowiczStanley M Spinola
Jun 26, 2009·The Journal of Infectious Diseases·Maria Labandeira-ReyEric J Hansen
Sep 2, 2010·Infection and Immunity·Maria Labandeira-ReyEric J Hansen
Jan 24, 2008·Infection and Immunity·Isabelle LeducChristopher Elkins
Dec 7, 2011·Infection and Immunity·Stanley M SpinolaRobert S Munson
Mar 24, 2005·Infection and Immunity·Catherine Dinitra WhiteChristopher Elkins
Mar 24, 2012·Future Microbiology·Jennifer A KalilJoanne M Langley
Apr 1, 2008·Microbiology·Margaret E BauerStanley M Spinola
Feb 10, 2009·The Journal of Infectious Diseases·Margaret E BauerDiane M Janowicz
Sep 18, 2013·Infection and Immunity·William G FuscoIsabelle Leduc
Jun 20, 2019·Frontiers in Microbiology·Ina MeuskensDirk Linke
Feb 23, 2021·Frontiers in Immunology·Julie A BrothwellStanley M Spinola

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