Expression of HCLCA1 in cystic fibrosis lungs is associated with mucus overproduction

The European Respiratory Journal
Hans-Peter HauberQ Hamid

Abstract

Mucus overproduction is typical in cystic fibrosis (CF) airway disease. The human calcium-activated chloride channel, hCLCA1, has been reported to be upregulated by interleukin (IL)-9 and to regulate the expression of mucins. Therefore, the expression of IL-9, IL-9 receptor (IL-9R) and hCLCA1 between the lungs of CF patients and healthy control subjects was compared. Endoscopic biopsy samples of bronchial mucosa from 10 CF patients and six control subjects were stained with periodic acid-Schiff. IL-9, IL-9R and hCLCA1 expression was determined by immunocytochemistry. Expression of hCLCA1 mRNA was also determined by in situ hybridisation. The present study found significant increases in IL-9, IL-9R and hCLCA1 immunoreactivity, hCLCA1 mRNA expression, and numbers of mucus-producing cells in the mucosa of CF patients compared to control subjects. Positive correlations were found between IL-9R-positive-cells with IL-9-positive cells and hCLCA1-positive cells, and between PAS-positive cells with hCLCA1-positive cells and IL-9R-positive cells. Expression of hCLCA1 mRNA was colocalised with IL-9R expression and PAS-positive staining in epithelial cells. Increased expression of interleukin-9 and interleukin-9 receptor, as well as an up...Continue Reading

Citations

Oct 29, 2008·Annual Review of Physiology·Anand C PatelMichael J Holtzman
Jun 11, 2005·American Journal of Respiratory Cell and Molecular Biology·Annette RobichaudSteven Xanthoudakis
Jan 21, 2006·American Journal of Respiratory Cell and Molecular Biology·S Marina Casalino-MatsudaRosanna M Forteza
Sep 2, 2008·American Journal of Respiratory Cell and Molecular Biology·S Marina Casalino-MatsudaRosanna M Forteza
Feb 27, 2009·American Journal of Respiratory Cell and Molecular Biology·Magali MeyerTeresinha Leal
Jun 9, 2009·American Journal of Respiratory Cell and Molecular Biology·Gerrit JohnMarkus O Henke
Mar 1, 2012·American Journal of Respiratory Cell and Molecular Biology·Lars MundhenkAchim D Gruber
Oct 17, 2008·Expert Opinion on Investigational Drugs·Sabina A Antoniu
Mar 25, 2009·The Journal of Physiology·Martine HamannMartin Main
Oct 23, 2009·British Journal of Pharmacology·Catherine M Greene, Noel G McElvaney
Dec 21, 2004·Pulmonary Pharmacology & Therapeutics·Duncan F Rogers
Jun 15, 2010·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Hans-Peter HauberQutayba Hamid
Mar 5, 2008·Journal of Occupational Medicine and Toxicology·Gaetano CaramoriAlberto Papi
Jul 15, 2004·Current Opinion in Pharmacology·Iain B McInnes, J Alastair Gracie
Jan 26, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y EndoA Nagai
May 28, 2005·The Journal of Biological Chemistry·Adele GibsonNicola Thompson
Jul 1, 2005·Physiological Reviews·Matthew E Loewen, George W Forsyth
Apr 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Kevin KimNorbert Perrimon
Dec 7, 2007·Gastroenterology·Fiona D YoungRichard F Rozmahel

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