PMID: 8586325Jan 1, 1994Paper

Expression of heat shock proteins in the normal and acutely stunned myocardium

Folia medica Cracoviensia
J Andres

Abstract

The aim was to examine the expression of ubiquitin (Ub), 27 kDa heat shock protein (hsp 27) and hsp 60 mRNA in normal and briefly ischemic and reperfused porcine myocardium. The left anterior descending coronary artery was occluded for two periods of 10 min, separated by 30 min of reperfusion. After the second occlusion the myocardium was reperfused up to 210 min. Tissue from ischemic, ischemic-reperfused and nonischemic regions of the heart were analysed by Northern and slot blot hybridization and nuclear run-on transcription assays employing radiolabelled cDNA probes for Ub, hsp 27 and hsp 60 as well as by Western blot using monoclonal antibodies recognizing Ub protein conjugates and antiserum recognizing hsp 27. Systolic wall thickening was significantly decreased at 30 min reperfusion after both occlusions and remained depressed at longer periods of reperfusion. Using Northern blot hybridizations several mRNAs encoding Ub, 0.9 kb mRNA encoding hsp 27, and 2.2 kb mRNA encoding hsp 60 were detected in sham-operated, nonischemic and ischemic myocardial tissues. Densitometric analysis of Northern and slot blot hybridization signals showed the significant increase of the basal tissue levels of Ub mRNA in stunned regions only dur...Continue Reading

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